Detailed information of TA system TA077514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 66051..66637
• Replicon: plasmid psymA
• Accession: NZ_CP021819
• Organism: Sinorhizobium meliloti strain M162

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO25_RS19275
• Protein ID: WP_088200862.1
• Coordinates: 66051..66440 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XCL0
• Locus tag: CDO25_RS19280
• Protein ID: WP_013851145.1
• Coordinates: 66437..66637 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO25_RS19255	61103..61780	-	678	WP_088200860.1	TIGR02594 family protein	-
CDO25_RS19260	62486..62893	-	408	WP_192936262.1	hypothetical protein	-
CDO25_RS19265	62912..64957	-	2046	WP_088200861.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
CDO25_RS19270	65021..65290	-	270	WP_192936263.1	hypothetical protein	-
CDO25_RS19275	66051..66440	-	390	WP_088200862.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO25_RS19280	66437..66637	-	201	WP_013851145.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CDO25_RS19285	66928..67329	+	402	WP_014531161.1	GFA family protein	-
CDO25_RS19290	67406..68242	-	837	WP_013851144.1	helix-turn-helix transcriptional regulator	-
CDO25_RS19295	68338..68661	+	324	WP_088200863.1	DUF1127 domain-containing protein	-
CDO25_RS19300	68700..69428	+	729	WP_013851143.1	class I SAM-dependent methyltransferase	-
CDO25_RS19305	69522..69668	+	147	WP_017266778.1	hypothetical protein	-
CDO25_RS19315	70235..71347	-	1113	WP_088197699.1	histidinol-phosphate transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	virB4	1..892745	892745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14503.80 Da
• Isoelectric Point: 6.6951

>T77514 WP_088200862.1 NZ_CP021819:c66440-66051 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPPIRGTEH

Nucleotide

• Length: 390 bp

>T77514 NZ_CP021819:c66440-66051 [Sinorhizobium meliloti]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTCATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCGCCCATACGCGGAACTGAACACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7096.22 Da
• Isoelectric Point: 11.1107

>AT77514 WP_013851145.1 NZ_CP021819:c66637-66437 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT77514 NZ_CP021819:c66637-66437 [Sinorhizobium meliloti]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7W6MID2