Detailed information of TA system TA077513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3101821..3102452
• Replicon: chromosome
• Accession: NZ_CP021818
• Organism: Sinorhizobium meliloti strain M162

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO25_RS15515
• Protein ID: WP_088200650.1
• Coordinates: 3101821..3102219 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92MD9
• Locus tag: CDO25_RS15520
• Protein ID: WP_010970129.1
• Coordinates: 3102219..3102452 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO25_RS15480	3096903..3097205	-	303	WP_010970122.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CDO25_RS15485	3097202..3097477	-	276	WP_010970123.1	type II toxin-antitoxin system ParD family antitoxin	-
CDO25_RS15490	3097763..3097912	+	150	WP_010970124.1	hypothetical protein	-
CDO25_RS15495	3097909..3098844	+	936	WP_010970125.1	site-specific tyrosine recombinase XerD	-
CDO25_RS15500	3098908..3099861	+	954	WP_003527479.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
CDO25_RS15505	3100106..3101548	+	1443	WP_010970126.1	murein L,D-transpeptidase	-
CDO25_RS15510	3101548..3101787	+	240	WP_010970127.1	sulfurtransferase TusA family protein	-
CDO25_RS15515	3101821..3102219	-	399	WP_088200650.1	type II toxin-antitoxin system toxin VapC	Toxin
CDO25_RS15520	3102219..3102452	-	234	WP_010970129.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDO25_RS15525	3102561..3103706	-	1146	WP_010970130.1	GTP-binding protein	-
CDO25_RS15530	3103720..3104802	-	1083	WP_010970131.1	D-alanyl-D-alanine carboxypeptidase	-
CDO25_RS15535	3105054..3106217	+	1164	WP_010970132.1	amidohydrolase	-
CDO25_RS15540	3106240..3106704	+	465	WP_010970133.1	Lrp/AsnC ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14770.06 Da
• Isoelectric Point: 6.6329

>T77513 WP_088200650.1 NZ_CP021818:c3102219-3101821 [Sinorhizobium meliloti]
MLTYMLDTNICIYVMKTYPPAVREKFNGLAEQLCISSITLGELHYGAEKSARRVENLTAIEHFVAPLEVLPFADKAAAHY
GQVRAELERTGTPCGPHDMQIGAHARSEGLIVVTNNIREFVRMPGVRVENWL

Nucleotide

• Length: 399 bp

>T77513 NZ_CP021818:c3102219-3101821 [Sinorhizobium meliloti]
ATGCTCACCTATATGCTCGATACCAATATCTGCATCTATGTGATGAAGACCTATCCGCCGGCCGTGCGCGAAAAGTTCAA
CGGACTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTGGGAGAATTACACTATGGCGCCGAAAAATCGGCCCGGCGCG
TTGAGAACCTTACGGCTATCGAACATTTCGTGGCACCGCTGGAGGTGCTGCCGTTCGCAGACAAGGCAGCGGCCCACTAC
GGGCAGGTTCGGGCTGAACTGGAGCGGACGGGAACGCCATGCGGCCCTCATGACATGCAGATCGGCGCGCATGCGCGCAG
CGAAGGGCTGATTGTCGTGACCAACAATATACGCGAGTTCGTCCGCATGCCGGGTGTAAGGGTCGAGAACTGGCTGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8673.87 Da
• Isoelectric Point: 8.9167

>AT77513 WP_010970129.1 NZ_CP021818:c3102452-3102219 [Sinorhizobium meliloti]
MASSTVFISNRSQAVRLPKAVAFPEGVHQVDILKIGRSRVIVPQGKRWDDLFLSGPRVSEDFMSERDQPVAETRESF

Nucleotide

• Length: 234 bp

>AT77513 NZ_CP021818:c3102452-3102219 [Sinorhizobium meliloti]
TTGGCCAGTTCGACCGTATTCATCAGTAACCGTAGCCAGGCGGTGCGGCTGCCAAAGGCCGTCGCCTTTCCCGAGGGCGT
GCATCAGGTCGATATCCTCAAGATCGGCCGCAGCCGCGTGATCGTCCCCCAGGGCAAAAGATGGGACGATCTGTTCCTCA
GCGGGCCGCGCGTCAGCGAGGATTTCATGAGCGAGCGTGATCAGCCGGTGGCGGAAACACGCGAATCCTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q92MD9