Detailed information of TA system TA077511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 2413991..2414645
• Replicon: chromosome
• Accession: NZ_CP021818
• Organism: Sinorhizobium meliloti strain M162

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: CDO25_RS12140
• Protein ID: WP_003530010.1
• Coordinates: 2413991..2414179 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: CDO25_RS12145
• Protein ID: WP_010969659.1
• Coordinates: 2414184..2414645 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO25_RS12125	2410557..2411867	+	1311	WP_088200601.1	HlyD family type I secretion periplasmic adaptor subunit	-
CDO25_RS12130	2411892..2412863	-	972	WP_017265202.1	glycosyltransferase family 2 protein	-
CDO25_RS12135	2413044..2413814	-	771	WP_010969658.1	hypothetical protein	-
CDO25_RS12140	2413991..2414179	+	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
CDO25_RS12145	2414184..2414645	+	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CDO25_RS12155	2414964..2415881	-	918	WP_003530005.1	PhzF family phenazine biosynthesis protein	-
CDO25_RS12160	2416058..2416807	-	750	WP_010969660.1	NUDIX hydrolase	-
CDO25_RS12165	2416810..2417916	-	1107	WP_003530001.1	endonuclease	-
CDO25_RS12170	2418067..2418510	-	444	WP_003529999.1	TIGR02594 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T77511 WP_003530010.1 NZ_CP021818:2413991-2414179 [Sinorhizobium meliloti]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T77511 NZ_CP021818:2413991-2414179 [Sinorhizobium meliloti]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT77511 WP_010969659.1 NZ_CP021818:2414184-2414645 [Sinorhizobium meliloti]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT77511 NZ_CP021818:2414184-2414645 [Sinorhizobium meliloti]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA

Structures

Toxin

• AlphaFold DB: H0G1A3

Antitoxin

• AlphaFold DB: Q92NX0