Detailed information of TA system TA077509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 531576..532141
• Replicon: chromosome
• Accession: NZ_CP021818
• Organism: Sinorhizobium meliloti strain M162

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: H0G3P4
• Locus tag: CDO25_RS02540
• Protein ID: WP_003531565.1
• Coordinates: 531896..532141 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: H0G3P5
• Locus tag: CDO25_RS02535
• Protein ID: WP_003531566.1
• Coordinates: 531576..531899 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO25_RS02515	527439..528239	+	801	WP_003531572.1	TVP38/TMEM64 family protein	-
CDO25_RS02520	528262..529683	+	1422	WP_088200289.1	NAD(P)/FAD-dependent oxidoreductase	-
CDO25_RS02525	529758..531278	+	1521	WP_010968584.1	HAMP domain-containing histidine kinase	-
CDO25_RS02535	531576..531899	-	324	WP_003531566.1	BrnA antitoxin family protein	Antitoxin
CDO25_RS02540	531896..532141	-	246	WP_003531565.1	BrnT family toxin	Toxin
CDO25_RS02545	532576..533289	+	714	WP_003531563.1	replicative DNA helicase	-
CDO25_RS34735	533348..533704	-	357	Protein_483	transposase	-
CDO25_RS02555	533849..534544	-	696	WP_015007123.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
CDO25_RS02560	534736..535224	+	489	WP_003531560.1	RidA family protein	-
CDO25_RS02565	535433..536050	+	618	WP_003531558.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	533348..533641	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-101)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 8990.48 Da
• Isoelectric Point: 9.7323

>T77509 WP_003531565.1 NZ_CP021818:c532141-531896 [Sinorhizobium meliloti]
MKVTYDETKRQINIGKHGYDIAELDMEFFASSVVVPAKEGRFMAIGVLRNGVIAVVFARLGSEGISIISMRDASRKERSL
L

Nucleotide

• Length: 246 bp

>T77509 NZ_CP021818:c532141-531896 [Sinorhizobium meliloti]
ATGAAGGTCACCTATGACGAAACAAAGCGCCAGATCAACATAGGAAAGCACGGCTATGATATCGCCGAACTCGACATGGA
GTTCTTCGCTTCGTCTGTGGTGGTCCCAGCCAAGGAAGGCCGCTTCATGGCTATCGGCGTTTTGCGCAATGGCGTTATCG
CCGTAGTCTTCGCCCGGCTTGGCTCGGAAGGCATTTCGATTATCTCCATGCGTGACGCCAGCAGGAAGGAAAGGAGCTTA
TTATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11933.51 Da
• Isoelectric Point: 10.2830

>AT77509 WP_003531566.1 NZ_CP021818:c531899-531576 [Sinorhizobium meliloti]
MTSLSRRIRHISDKEEAAIQRKIASDPDAPEATDEQLAKAKPFAEAFPDLAESIKRARGRPAVENPRQQISIRLDPDVIE
HYKATGKGWQSRMNDDLRKAAGLKKAG

Nucleotide

• Length: 324 bp

>AT77509 NZ_CP021818:c531899-531576 [Sinorhizobium meliloti]
ATGACCAGCCTATCCCGCCGCATCCGCCATATCTCCGACAAGGAGGAGGCAGCTATCCAACGCAAGATAGCTAGTGACCC
AGACGCTCCTGAGGCCACCGACGAACAACTAGCCAAAGCTAAGCCATTCGCCGAAGCCTTCCCAGACCTGGCCGAGAGCA
TCAAGCGCGCCAGGGGCCGTCCAGCCGTTGAGAACCCTAGGCAGCAGATATCCATTCGCCTCGATCCGGATGTCATCGAG
CACTACAAGGCAACCGGCAAAGGCTGGCAGAGCCGGATGAATGACGACCTTAGAAAGGCTGCCGGATTGAAGAAGGCAGG
TTGA

Structures

Toxin

• AlphaFold DB: H0G3P4

Antitoxin

• AlphaFold DB: H0G3P5