77508

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4648..5309 Replicon chromosome
Accession NZ_CP021818
Organism Sinorhizobium meliloti strain M162

Toxin (Protein)


Gene name vapC Uniprot ID Q92KY5
Locus tag CDO25_RS00025 Protein ID WP_010970565.1
Coordinates 4648..5052 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q92KY4
Locus tag CDO25_RS00030 Protein ID WP_010970566.1
Coordinates 5049..5309 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO25_RS00010 191..1303 + 1113 WP_003535893.1 3-isopropylmalate dehydrogenase -
CDO25_RS00015 1432..2139 + 708 WP_010970563.1 phosphatase PAP2 family protein -
CDO25_RS00020 2240..4129 - 1890 WP_014527006.1 MHS family MFS transporter -
CDO25_RS00025 4648..5052 - 405 WP_010970565.1 type II toxin-antitoxin system VapC family toxin Toxin
CDO25_RS00030 5049..5309 - 261 WP_010970566.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CDO25_RS00035 5438..5608 + 171 WP_010970567.1 hypothetical protein -
CDO25_RS00040 5722..6756 + 1035 WP_003531790.1 aspartate-semialdehyde dehydrogenase -
CDO25_RS00045 6929..7750 + 822 WP_013844939.1 lytic transglycosylase family protein -
CDO25_RS00055 8097..8780 - 684 WP_010970569.1 carbonic anhydrase -
CDO25_RS00060 8954..9835 - 882 WP_010970570.1 pyridoxal kinase PdxY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(8-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14507.71 Da        Isoelectric Point: 6.7257

>T77508 WP_010970565.1 NZ_CP021818:c5052-4648 [Sinorhizobium meliloti]
VISHILDTNAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Download         Length: 405 bp

>T77508 NZ_CP021818:c5052-4648 [Sinorhizobium meliloti]
GTGATCTCCCATATTCTCGACACCAACGCCGTCATCGCTTTGATCGGGCGCAAATCGGATGCATTGGTCACCCGTGTACT
CCATAGCCCTCAGGGCATTATCGGGCTCCCGTCGGTCGTCGCGTATGAACTCTATTTTGGAGCGCAGAAAAGCGCGAAGG
CCCAACACAATCTCGAAACCCTGCGTTTGTTGATGGCGGATTTTCCGATCCTCGACTTCGACCGGAACGATGCCTTTGTC
GCAGGCGAGATCCGGGCCGCCCTTGCCGCGAAGGGTACGCCCATCGGACCTTATGACGTGCTGATTGCGGGACAGGCCAA
AGCCCGTGGATTGACGCTCGTCACCAACAATGTCGGCGAGTTCAATAGGGTTGAGAACCTGCGCGTGGAGGATTGGTCGC
TCTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9903.16 Da        Isoelectric Point: 5.1253

>AT77508 WP_010970566.1 NZ_CP021818:c5309-5049 [Sinorhizobium meliloti]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRSE
LDAVFR

Download         Length: 261 bp

>AT77508 NZ_CP021818:c5309-5049 [Sinorhizobium meliloti]
ATGCCCCATCTTGCACGGGTGTTCCAGTCCGGAAATTCGCAGGCCGTCCGCCTGCCTAAGGAATTCCGCTTCAACGTCGA
TCGCGTCGAAATCACGCAGGAAGGCGACGCGCTCATTCTCCGCCCGCACGTCGAGCAAGGCGAGCAGTGGTCATCGCTGA
AGGCGGCACTTGCCCGTGGCATGAGTGAAGACTTCATGATGTGCGGGCGCGAGCAGCCGGAACAGCAGGATCGGTCGGAA
CTCGACGCGGTATTTCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92KY5

Antitoxin

Source ID Structure
AlphaFold DB Q92KY4

References