Detailed information of TA system TA077506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1547583..1548117
• Replicon: plasmid psymB
• Accession: NZ_CP021814
• Organism: Sinorhizobium meliloti strain M270

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CDO26_RS33235
• Protein ID: WP_088198797.1
• Coordinates: 1547583..1547879 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: CDO26_RS33240
• Protein ID: WP_003527850.1
• Coordinates: 1547869..1548117 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO26_RS33205 (CDO26_33185)	1543073..1544452	-	1380	WP_027991363.1	sodium:alanine symporter family protein	-
CDO26_RS33210 (CDO26_33190)	1544974..1545183	-	210	WP_003527838.1	dodecin family protein	-
CDO26_RS33215 (CDO26_33195)	1545473..1545919	+	447	WP_014527651.1	DNA-binding protein	-
CDO26_RS33220 (CDO26_33200)	1545923..1546108	-	186	WP_014527652.1	hypothetical protein	-
CDO26_RS33225 (CDO26_33205)	1546275..1546673	-	399	WP_017269012.1	hypothetical protein	-
CDO26_RS33230 (CDO26_33210)	1547246..1547508	+	263	Protein_1438	helix-turn-helix domain-containing protein	-
CDO26_RS33235 (CDO26_33215)	1547583..1547879	-	297	WP_088198797.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO26_RS33240 (CDO26_33220)	1547869..1548117	-	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CDO26_RS33255 (CDO26_33235)	1548735..1548860	+	126	Protein_1441	IS481 family transposase	-
CDO26_RS33260 (CDO26_33240)	1548944..1549738	-	795	WP_014527654.1	methyltransferase domain-containing protein	-
CDO26_RS33265 (CDO26_33245)	1549742..1550542	-	801	WP_014527655.1	ABC transporter ATP-binding protein	-
CDO26_RS33270 (CDO26_33250)	1550539..1551582	-	1044	WP_088198798.1	iron ABC transporter permease	-
CDO26_RS33275 (CDO26_33255)	1551579..1552646	-	1068	WP_010974979.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1694441	1694441
	-	inside	IScluster/Tn	-	-	1547246..1548860	1614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-93)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11462.14 Da
• Isoelectric Point: 10.8417

>T77506 WP_088198797.1 NZ_CP021814:c1547879-1547583 [Sinorhizobium meliloti]
MATERPFRLAPAAKADLRKIWRYTARRWSLEQAETYQDQLYTAFEGLAAGTKKGRNVDVRPGYLKYPAGSHIVYFRNRGD
RIDIIRILHGRMDAQRHL

Nucleotide

• Length: 297 bp

>T77506 NZ_CP021814:c1547879-1547583 [Sinorhizobium meliloti]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTGCCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGCAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGACCGGGTTATCTCAAATACCCGGCAGGATCGCACATCGTCTATTTTCGGAATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGGATGGATGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT77506 WP_003527850.1 NZ_CP021814:c1548117-1547869 [Sinorhizobium meliloti]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT77506 NZ_CP021814:c1548117-1547869 [Sinorhizobium meliloti]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CTTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Antitoxin

• AlphaFold DB: H0FXK0