Detailed information of TA system TA077503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1079099..1079784
• Replicon: plasmid psymA
• Accession: NZ_CP021813
• Organism: Sinorhizobium meliloti strain M270

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO26_RS23745
• Protein ID: WP_088198215.1
• Coordinates: 1079099..1079518 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CDO26_RS23750
• Protein ID: WP_088196198.1
• Coordinates: 1079515..1079784 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO26_RS23720 (CDO26_23725)	1074752..1075144	+	393	WP_088198213.1	c-type cytochrome	-
CDO26_RS23725 (CDO26_23730)	1075222..1075692	+	471	WP_015242247.1	(2Fe-2S)-binding protein	-
CDO26_RS23730 (CDO26_23735)	1075703..1077874	+	2172	WP_088198214.1	molybdopterin-dependent oxidoreductase	-
CDO26_RS39175	1078611..1078769	+	159	WP_164820653.1	hypothetical protein	-
CDO26_RS23745 (CDO26_23750)	1079099..1079518	-	420	WP_088198215.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO26_RS23750 (CDO26_23755)	1079515..1079784	-	270	WP_088196198.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDO26_RS23755 (CDO26_23760)	1079915..1080381	+	467	Protein_1102	LysR substrate-binding domain-containing protein	-
CDO26_RS23760 (CDO26_23765)	1080472..1081347	-	876	WP_164820652.1	LysR family transcriptional regulator	-
CDO26_RS23770 (CDO26_23775)	1081810..1082019	+	210	WP_234837684.1	hypothetical protein	-
CDO26_RS23775 (CDO26_23780)	1082076..1082456	-	381	WP_088198216.1	transcriptional regulator	-
CDO26_RS40140	1082906..1083262	-	357	WP_234896313.1	hypothetical protein	-
CDO26_RS23785 (CDO26_23785)	1083171..1083830	-	660	WP_234896311.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1454983	1454983

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14837.05 Da
• Isoelectric Point: 4.5732

>T77503 WP_088198215.1 NZ_CP021813:c1079518-1079099 [Sinorhizobium meliloti]
VSRLYMLDTDIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILESMQVLALDVPADAE
YGGIRAELEAAGRPIGPNDLFIAAHACMLGAVLVTANSSEFTRIRDLKVENWLDFTSSG

Nucleotide

• Length: 420 bp

>T77503 NZ_CP021813:c1079518-1079099 [Sinorhizobium meliloti]
GTGAGCAGGCTCTACATGCTGGACACCGATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTGCTGGCGCAAATCGAGACCATTCTTGAAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGGAGGCCCATCGGCCCGAATGACCTGTTCATCGCGGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCATTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTTCTTCGGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10202.68 Da
• Isoelectric Point: 4.5716

>AT77503 WP_088196198.1 NZ_CP021813:c1079784-1079515 [Sinorhizobium meliloti]
MPQLRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT77503 NZ_CP021813:c1079784-1079515 [Sinorhizobium meliloti]
ATGCCCCAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCCAAGCTCTTCCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAAGTGCTGGCTGGTTTGGAGCCGCTTGGGTCTGAAGATGAGTTCCCTGACGTTGACGAAACC
CTGTTGCCGGTGAAGGAAATCGACCTGTGA