Detailed information of TA system TA077497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3182421..3183082
• Replicon: chromosome
• Accession: NZ_CP021812
• Organism: Sinorhizobium meliloti strain M270

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92KY5
• Locus tag: CDO26_RS16570
• Protein ID: WP_010970565.1
• Coordinates: 3182678..3183082 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92KY4
• Locus tag: CDO26_RS16565
• Protein ID: WP_010970566.1
• Coordinates: 3182421..3182681 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO26_RS16535 (CDO26_16545)	3177895..3178776	+	882	WP_010970570.1	pyridoxal kinase PdxY	-
CDO26_RS16540 (CDO26_16550)	3178950..3179633	+	684	WP_010970569.1	carbonic anhydrase	-
CDO26_RS16550 (CDO26_16560)	3179980..3180801	-	822	WP_013844939.1	lytic transglycosylase domain-containing protein	-
CDO26_RS16555 (CDO26_16565)	3180974..3182008	-	1035	WP_003531790.1	aspartate-semialdehyde dehydrogenase	-
CDO26_RS16560 (CDO26_16570)	3182122..3182292	-	171	WP_010970567.1	hypothetical protein	-
CDO26_RS16565 (CDO26_16575)	3182421..3182681	+	261	WP_010970566.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDO26_RS16570 (CDO26_16580)	3182678..3183082	+	405	WP_010970565.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO26_RS16575 (CDO26_16585)	3183601..3185490	+	1890	WP_014527006.1	MFS transporter	-
CDO26_RS16580 (CDO26_16590)	3185591..3186298	-	708	WP_010970563.1	phosphatase PAP2 family protein	-
CDO26_RS16585 (CDO26_16595)	3186427..3187539	-	1113	WP_088197394.1	3-isopropylmalate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (8-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14507.71 Da
• Isoelectric Point: 6.7257

>T77497 WP_010970565.1 NZ_CP021812:3182678-3183082 [Sinorhizobium meliloti]
VISHILDTNAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Nucleotide

• Length: 405 bp

>T77497 NZ_CP021812:3182678-3183082 [Sinorhizobium meliloti]
GTGATCTCCCATATTCTCGACACCAACGCCGTCATCGCTTTGATCGGGCGCAAATCGGATGCATTGGTCACCCGTGTACT
CCATAGCCCTCAGGGCATTATCGGGCTCCCGTCGGTCGTCGCGTATGAACTCTATTTTGGAGCGCAGAAAAGCGCGAAGG
CCCAACACAATCTCGAAACCCTGCGTTTGTTGATGGCGGATTTTCCGATCCTCGACTTCGACCGGAACGATGCCTTTGTC
GCAGGCGAGATCCGGGCCGCCCTTGCCGCGAAGGGTACGCCCATCGGACCTTATGACGTGCTGATTGCGGGACAGGCCAA
AGCCCGTGGATTGACGCTCGTCACCAACAATGTCGGCGAGTTCAATAGGGTTGAGAACCTGCGCGTGGAGGATTGGTCGC
TCTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9903.16 Da
• Isoelectric Point: 5.1253

>AT77497 WP_010970566.1 NZ_CP021812:3182421-3182681 [Sinorhizobium meliloti]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRSE
LDAVFR

Nucleotide

• Length: 261 bp

>AT77497 NZ_CP021812:3182421-3182681 [Sinorhizobium meliloti]
ATGCCCCATCTTGCACGGGTGTTCCAGTCCGGAAATTCGCAGGCCGTCCGCCTGCCTAAGGAATTCCGCTTCAACGTCGA
TCGCGTCGAAATCACGCAGGAAGGCGACGCGCTCATTCTCCGCCCGCACGTCGAGCAAGGCGAGCAGTGGTCATCGCTGA
AGGCGGCACTTGCCCGTGGCATGAGTGAAGACTTCATGATGTGCGGGCGCGAGCAGCCGGAACAGCAGGATCGGTCGGAA
CTCGACGCGGTATTTCGGTGA

Structures

Toxin

• AlphaFold DB: Q92KY5

Antitoxin

• AlphaFold DB: Q92KY4