77496

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 2654546..2655111 Replicon chromosome
Accession NZ_CP021812
Organism Sinorhizobium meliloti strain M270

Toxin (Protein)


Gene name brnT Uniprot ID H0G3P4
Locus tag CDO26_RS14040 Protein ID WP_003531565.1
Coordinates 2654546..2654791 (+) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID H0G3P5
Locus tag CDO26_RS14045 Protein ID WP_003531566.1
Coordinates 2654788..2655111 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO26_RS14015 (CDO26_14025) 2649777..2650922 - 1146 WP_003531550.1 alpha-hydroxy acid oxidase -
CDO26_RS14020 (CDO26_14030) 2651010..2651627 - 618 WP_003531558.1 hypothetical protein -
CDO26_RS14025 (CDO26_14035) 2651836..2652324 - 489 WP_003531560.1 RidA family protein -
CDO26_RS14030 (CDO26_14040) 2652516..2653211 + 696 WP_015007123.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
CDO26_RS14035 (CDO26_14045) 2653398..2654111 - 714 WP_003531563.1 DNA helicase -
CDO26_RS14040 (CDO26_14050) 2654546..2654791 + 246 WP_003531565.1 BrnT family toxin Toxin
CDO26_RS14045 (CDO26_14055) 2654788..2655111 + 324 WP_003531566.1 BrnA antitoxin family protein Antitoxin
CDO26_RS14055 (CDO26_14065) 2655409..2656929 - 1521 WP_167693500.1 HAMP domain-containing sensor histidine kinase -
CDO26_RS14060 (CDO26_14070) 2657004..2658425 - 1422 WP_003531570.1 NAD(P)/FAD-dependent oxidoreductase -
CDO26_RS14065 (CDO26_14075) 2658448..2659248 - 801 WP_088197343.1 TVP38/TMEM64 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-77)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-101)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 8990.48 Da        Isoelectric Point: 9.7323

>T77496 WP_003531565.1 NZ_CP021812:2654546-2654791 [Sinorhizobium meliloti]
MKVTYDETKRQINIGKHGYDIAELDMEFFASSVVVPAKEGRFMAIGVLRNGVIAVVFARLGSEGISIISMRDASRKERSL
L

Download         Length: 246 bp

>T77496 NZ_CP021812:2654546-2654791 [Sinorhizobium meliloti]
ATGAAGGTCACCTATGACGAAACAAAGCGCCAGATCAACATAGGAAAGCACGGCTATGATATCGCCGAACTCGACATGGA
GTTCTTCGCTTCGTCTGTGGTGGTCCCAGCCAAGGAAGGCCGCTTCATGGCTATCGGCGTTTTGCGCAATGGCGTTATCG
CCGTAGTCTTCGCCCGGCTTGGCTCGGAAGGCATTTCGATTATCTCCATGCGTGACGCCAGCAGGAAGGAAAGGAGCTTA
TTATGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 11933.51 Da        Isoelectric Point: 10.2830

>AT77496 WP_003531566.1 NZ_CP021812:2654788-2655111 [Sinorhizobium meliloti]
MTSLSRRIRHISDKEEAAIQRKIASDPDAPEATDEQLAKAKPFAEAFPDLAESIKRARGRPAVENPRQQISIRLDPDVIE
HYKATGKGWQSRMNDDLRKAAGLKKAG

Download         Length: 324 bp

>AT77496 NZ_CP021812:2654788-2655111 [Sinorhizobium meliloti]
ATGACCAGCCTATCCCGCCGCATCCGCCATATCTCCGACAAGGAGGAGGCAGCTATCCAACGCAAGATAGCTAGTGACCC
AGACGCTCCTGAGGCCACCGACGAACAACTAGCCAAAGCTAAGCCATTCGCCGAAGCCTTCCCAGACCTGGCCGAGAGCA
TCAAGCGCGCCAGGGGCCGTCCAGCCGTTGAGAACCCTAGGCAGCAGATATCCATTCGCCTCGATCCGGATGTCATCGAG
CACTACAAGGCAACCGGCAAAGGCTGGCAGAGCCGGATGAATGACGACCTTAGAAAGGCTGCCGGATTGAAGAAGGCAGG
TTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H0G3P4

Antitoxin

Source ID Structure
AlphaFold DB H0G3P5

References