Detailed information of TA system TA077495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2274781..2275421
• Replicon: chromosome
• Accession: NZ_CP021812
• Organism: Sinorhizobium meliloti strain M270

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H0G6N1
• Locus tag: CDO26_RS12175
• Protein ID: WP_003533691.1
• Coordinates: 2275035..2275421 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0G6N0
• Locus tag: CDO26_RS12170
• Protein ID: WP_003533689.1
• Coordinates: 2274781..2275035 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO26_RS12150 (CDO26_12160)	2270088..2270324	-	237	WP_003533684.1	DUF4170 domain-containing protein	-
CDO26_RS12155 (CDO26_12165)	2270385..2271203	-	819	WP_010968835.1	3'(2'),5'-bisphosphate nucleotidase CysQ	-
CDO26_RS12160 (CDO26_12170)	2271312..2272658	-	1347	WP_010968834.1	TldD/PmbA family protein	-
CDO26_RS12165 (CDO26_12175)	2272810..2274642	+	1833	WP_010968833.1	monovalent cation:proton antiporter-2 (CPA2) family protein	-
CDO26_RS12170 (CDO26_12180)	2274781..2275035	+	255	WP_003533689.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CDO26_RS12175 (CDO26_12185)	2275035..2275421	+	387	WP_003533691.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO26_RS12180 (CDO26_12190)	2275650..2276549	-	900	WP_010968832.1	patatin-like phospholipase family protein	-
CDO26_RS12185 (CDO26_12195)	2276622..2277182	-	561	WP_010968831.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
CDO26_RS12190 (CDO26_12200)	2277189..2278910	-	1722	WP_013844081.1	pseudouridine synthase	-
CDO26_RS12195 (CDO26_12205)	2278985..2279434	+	450	WP_003537305.1	nucleoside deaminase	-
CDO26_RS12200 (CDO26_12210)	2279478..2279957	-	480	WP_003537307.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13904.77 Da
• Isoelectric Point: 4.5716

>T77495 WP_003533691.1 NZ_CP021812:2275035-2275421 [Sinorhizobium meliloti]
MVIDTSAIAAIAFNEQEAGSFREKIADDPVRLISAATALEAAMVIETRLGEAAGAELDLWLYKANVEIVAVTAEHMDQAR
RAWRRFGKGRHPAGLNFGDCFSYALAFLTNEPLLFKGSDFSQTDIPAA

Nucleotide

• Length: 387 bp

>T77495 NZ_CP021812:2275035-2275421 [Sinorhizobium meliloti]
ATGGTCATCGACACATCCGCCATTGCCGCGATTGCCTTCAATGAACAGGAGGCCGGAAGTTTCCGCGAAAAAATCGCGGA
CGACCCGGTTCGGCTCATCTCCGCGGCAACCGCTCTGGAAGCGGCCATGGTAATCGAAACCCGGTTAGGCGAAGCTGCCG
GAGCGGAACTTGATCTTTGGCTTTACAAGGCAAACGTGGAGATTGTTGCGGTAACCGCCGAGCATATGGACCAGGCCCGC
CGTGCCTGGCGCCGTTTCGGCAAAGGCCGTCATCCGGCCGGTTTGAATTTCGGTGATTGTTTTTCCTATGCCCTGGCCTT
TCTGACAAATGAACCGCTTCTCTTCAAGGGAAGCGATTTCTCGCAAACCGATATCCCGGCGGCATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9472.63 Da
• Isoelectric Point: 4.8660

>AT77495 WP_003533689.1 NZ_CP021812:2274781-2275035 [Sinorhizobium meliloti]
MALSIKDTETEQLARTLARRTGESITLATKRALEERLRRTDTAARRAALLEDLEAIQRRWNAMPVLDSRSSDDIIGYDED
GLPS

Nucleotide

• Length: 255 bp

>AT77495 NZ_CP021812:2274781-2275035 [Sinorhizobium meliloti]
ATGGCGCTGAGCATCAAGGACACTGAAACCGAGCAGCTTGCCCGCACACTTGCCCGGCGGACAGGGGAATCCATCACGCT
TGCCACCAAGCGTGCCCTCGAAGAACGATTGCGCCGAACCGACACCGCTGCCCGGCGCGCGGCTCTTCTGGAGGATCTGG
AAGCCATTCAACGCCGCTGGAATGCCATGCCCGTCCTCGACAGTCGCAGCTCCGATGACATTATCGGTTATGACGAGGAT
GGACTCCCCTCATAA

Structures

Toxin

• AlphaFold DB: H0G6N1

Antitoxin

• AlphaFold DB: H0G6N0