Detailed information of TA system TA077491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 775825..776359
• Replicon: plasmid psymB
• Accession: NZ_CP021810
• Organism: Sinorhizobium meliloti strain Rm41

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q92X99
• Locus tag: CDO27_RS30325
• Protein ID: WP_010974986.1
• Coordinates: 775825..776121 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: CDO27_RS30330
• Protein ID: WP_003527850.1
• Coordinates: 776111..776359 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO27_RS30295	771267..772670	-	1404	WP_026029937.1	sodium:alanine symporter family protein	-
CDO27_RS30300	773204..773413	-	210	WP_003527838.1	dodecin family protein	-
CDO27_RS30305	773703..774149	+	447	WP_014527651.1	DNA-binding protein	-
CDO27_RS30310	774153..774338	-	186	WP_010974989.1	hypothetical protein	-
CDO27_RS30315	774505..774903	-	399	WP_010974988.1	hypothetical protein	-
CDO27_RS30320	775484..775744	+	261	Protein_717	helix-turn-helix domain containing protein	-
CDO27_RS30325	775825..776121	-	297	WP_010974986.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO27_RS30330	776111..776359	-	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CDO27_RS30345	776977..777102	+	126	Protein_720	IS481 family transposase	-
CDO27_RS30350	777186..777980	-	795	WP_013850206.1	methyltransferase domain-containing protein	-
CDO27_RS30355	777984..778784	-	801	WP_015008735.1	ABC transporter ATP-binding protein	-
CDO27_RS30360	778781..779824	-	1044	WP_010974980.1	iron ABC transporter permease	-
CDO27_RS30365	779821..780906	-	1086	WP_015008736.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1665079	1665079
	-	flank	IS/Tn	-	-	776911..777102	191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-91)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11473.15 Da
• Isoelectric Point: 10.6593

>T77491 WP_010974986.1 NZ_CP021810:c776121-775825 [Sinorhizobium meliloti]
MATERPFRLAPTAKADLRKIWRYTARRWSLEQAETYQDQLYTAFEGLAVGTKKGRNVDVRPGYLKYPAGAHIVYFRDRGD
RIDIIRILHGRVDAQRHL

Nucleotide

• Length: 297 bp

>T77491 NZ_CP021810:c776121-775825 [Sinorhizobium meliloti]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTACCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGTAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGTGTGGACGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT77491 WP_003527850.1 NZ_CP021810:c776359-776111 [Sinorhizobium meliloti]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT77491 NZ_CP021810:c776359-776111 [Sinorhizobium meliloti]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CCTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Toxin

• AlphaFold DB: Q92X99

Antitoxin

• AlphaFold DB: H0FXK0