Detailed information of TA system TA077490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 423413..424080
• Replicon: plasmid psymB
• Accession: NZ_CP021810
• Organism: Sinorhizobium meliloti strain Rm41

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO27_RS28595
• Protein ID: WP_015008546.1
• Coordinates: 423637..424080 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92WE1
• Locus tag: CDO27_RS28590
• Protein ID: WP_010975287.1
• Coordinates: 423413..423640 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO27_RS28565	418806..419687	-	882	WP_003525869.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
CDO27_RS28570	419805..421097	-	1293	WP_015008543.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
CDO27_RS28580	421467..422756	+	1290	WP_015008544.1	SDR family oxidoreductase	-
CDO27_RS28585	422756..423229	+	474	WP_015008545.1	DUF2269 family protein	-
CDO27_RS28590	423413..423640	+	228	WP_010975287.1	hypothetical protein	Antitoxin
CDO27_RS28595	423637..424080	+	444	WP_015008546.1	PIN domain-containing protein	Toxin
CDO27_RS28600	424096..425034	-	939	WP_015008547.1	choline ABC transporter substrate-binding protein	-
CDO27_RS28605	425103..425933	-	831	WP_013849981.1	SDR family oxidoreductase	-
CDO27_RS28610	425930..426427	-	498	WP_015008548.1	acetone carboxylase subunit gamma	-
CDO27_RS28615	426431..428623	-	2193	WP_015008549.1	hydantoinase B/oxoprolinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1665079	1665079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15649.96 Da
• Isoelectric Point: 6.9771

>T77490 WP_015008546.1 NZ_CP021810:423637-424080 [Sinorhizobium meliloti]
VTFLLDVNVLIALIDPSHIGHNDAHEWFASIGQTTWATCPITENGVIRIVGNPEYPNSPGSPLPVMEIVAKLRSLPGHVF
WPDDVSLVGSSDIIPSKILTSGQVTDTYLLALAKARGGKLATFDRKLSAAAVTKGNSALHLIATNRS

Nucleotide

• Length: 444 bp

>T77490 NZ_CP021810:423637-424080 [Sinorhizobium meliloti]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGAGCCATATAGGCCATAATGATGCGCACGAATG
GTTCGCGTCGATTGGTCAGACCACGTGGGCCACCTGCCCAATTACCGAAAATGGTGTCATCCGGATCGTCGGCAATCCCG
AATATCCCAATTCTCCCGGCTCACCGTTACCCGTGATGGAGATCGTCGCCAAATTGCGGTCGCTCCCCGGTCATGTTTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCAAGTGATATCATCCCATCGAAAATCCTGACTTCGGGGCAGGTGACTGACAC
CTATCTGCTTGCGCTCGCCAAGGCGCGTGGGGGCAAGCTGGCGACATTCGATCGCAAGCTGTCGGCAGCGGCGGTCACGA
AGGGCAATTCCGCCTTGCACCTGATCGCGACGAATAGGTCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8174.39 Da
• Isoelectric Point: 9.1096

>AT77490 WP_010975287.1 NZ_CP021810:423413-423640 [Sinorhizobium meliloti]
MRTTLAIDDDVLIAAKAMATQQQRSVGEVISELARRSLRRPRSGGERNGIPLLSPRPDAPPVTLEIVNALRDELA

Nucleotide

• Length: 228 bp

>AT77490 NZ_CP021810:423413-423640 [Sinorhizobium meliloti]
ATGAGAACGACCTTGGCTATTGACGACGATGTCCTGATCGCCGCAAAGGCAATGGCGACCCAACAGCAGCGGAGCGTCGG
CGAGGTAATTTCCGAACTGGCGAGGCGTTCTCTGCGCCGGCCGCGCAGCGGCGGCGAGCGTAATGGAATTCCTCTCCTGT
CGCCTCGTCCGGACGCACCACCCGTGACGCTCGAGATCGTCAACGCCCTGCGTGACGAACTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: Q92WE1