Detailed information of TA system TA077489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 75258..76190
• Replicon: plasmid psymB
• Accession: NZ_CP021810
• Organism: Sinorhizobium meliloti strain Rm41

Toxin (Protein)

• Gene name: -
• Uniprot ID: F7XGP1
• Locus tag: CDO27_RS26845
• Protein ID: WP_014530511.1
• Coordinates: 75258..75812 (-)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: F7XGP2
• Locus tag: CDO27_RS26850
• Protein ID: WP_014530512.1
• Coordinates: 75819..76190 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO27_RS26825	70787..71653	+	867	WP_015008365.1	sulfate ABC transporter permease subunit CysT	-
CDO27_RS26830	71643..72518	+	876	WP_013850796.1	sulfate ABC transporter permease subunit CysW	-
CDO27_RS26835	72534..73640	+	1107	WP_015008366.1	sulfate/molybdate ABC transporter ATP-binding protein	-
CDO27_RS26840	73752..75122	+	1371	WP_015008367.1	FAD-binding oxidoreductase	-
CDO27_RS26845	75258..75812	-	555	WP_014530511.1	RES family NAD+ phosphorylase	Toxin
CDO27_RS26850	75819..76190	-	372	WP_014530512.1	DUF2384 domain-containing protein	Antitoxin
CDO27_RS26855	76267..77052	-	786	WP_014530513.1	crotonase/enoyl-CoA hydratase family protein	-
CDO27_RS26860	77049..77918	-	870	WP_014530514.1	hydroxymethylglutaryl-CoA lyase	-
CDO27_RS26865	77915..79903	-	1989	WP_015008368.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
CDO27_RS26870	79920..80303	-	384	WP_010975573.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1665079	1665079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (23-177)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20449.31 Da
• Isoelectric Point: 6.4901

>T77489 WP_014530511.1 NZ_CP021810:c75812-75258 [Sinorhizobium meliloti]
MRKHIPPASRPDSEPTSPVEPITLWRAFVPRWAHMPLSGAGAARFGGRWNPIGMPAIYAARELSTAWAEYNQGFVQHPAL
IVQLELRGARLADLTDASVLLELGVDEAIHRCEWRDALDKGAVPETHRLQSELLARDYHGVIYPSFMSPGGTCVALWRWN
GAEEPRLDVIDPDGRLPKSPASWV

Nucleotide

• Length: 555 bp

>T77489 NZ_CP021810:c75812-75258 [Sinorhizobium meliloti]
ATGAGGAAACACATTCCGCCCGCATCGCGACCCGATTCGGAACCGACGTCGCCGGTCGAGCCGATAACGCTCTGGCGCGC
CTTCGTTCCGCGCTGGGCACATATGCCGCTATCCGGCGCGGGTGCCGCTCGCTTCGGTGGCCGCTGGAACCCGATCGGCA
TGCCTGCGATTTATGCTGCGCGCGAGCTTTCCACTGCCTGGGCTGAATATAATCAGGGTTTCGTCCAGCATCCGGCGCTC
ATCGTGCAGCTCGAGTTGCGCGGGGCGAGGCTCGCGGACCTCACGGATGCCAGCGTTCTTCTGGAGCTTGGTGTGGATGA
GGCGATCCATCGCTGCGAATGGCGTGATGCGCTCGACAAAGGCGCGGTGCCGGAGACCCACAGGCTGCAGTCGGAGCTTC
TGGCGCGCGACTATCACGGGGTGATCTATCCATCCTTCATGTCGCCCGGCGGCACCTGCGTCGCGCTCTGGCGCTGGAAC
GGTGCGGAAGAACCGCGTCTCGACGTCATCGATCCCGATGGCCGGCTGCCGAAATCGCCCGCCTCATGGGTTTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13117.25 Da
• Isoelectric Point: 9.6233

>AT77489 WP_014530512.1 NZ_CP021810:c76190-75819 [Sinorhizobium meliloti]
MMAVDFQIAAARFGDDHSPFLSARLVADWLGVTLAELAKLIGVARNTLTAKSGARKVDSALSKVVRILAMASEMAGDEAR
AVIWFKHQPIPGWAGKTAFDLVGEGKADKVLAYLESVRAGVYA

Nucleotide

• Length: 372 bp

>AT77489 NZ_CP021810:c76190-75819 [Sinorhizobium meliloti]
ATGATGGCCGTTGATTTCCAGATAGCTGCAGCCCGTTTCGGGGACGATCATTCCCCGTTTCTCTCGGCCCGCCTTGTCGC
CGACTGGTTGGGCGTCACGCTCGCCGAACTCGCCAAGCTGATCGGTGTCGCCCGCAATACGCTGACGGCGAAATCCGGCG
CCCGGAAGGTCGACAGCGCCTTGAGCAAGGTCGTCCGCATCCTCGCCATGGCTTCCGAAATGGCGGGCGACGAAGCCCGC
GCCGTCATCTGGTTCAAGCACCAGCCTATCCCCGGCTGGGCCGGCAAGACGGCGTTCGATCTGGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAATCGGTACGTGCCGGCGTCTACGCTTAA

Structures

Toxin

• AlphaFold DB: A0A222K2B4

Antitoxin

• AlphaFold DB: A0A222K374