Detailed information of TA system TA077486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 559058..559593
• Replicon: plasmid psymA
• Accession: NZ_CP021809
• Organism: Sinorhizobium meliloti strain Rm41

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CDO27_RS21270
• Protein ID: WP_014989489.1
• Coordinates: 559318..559593 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N8JT21
• Locus tag: CDO27_RS21265
• Protein ID: WP_013845222.1
• Coordinates: 559058..559318 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO27_RS21235	554500..556467	-	1968	WP_014989492.1	AAA family ATPase	-
CDO27_RS21245	556657..557805	-	1149	WP_041169439.1	DUF1612 and helix-turn-helix domain-containing protein	-
CDO27_RS21250	558028..558270	+	243	WP_013845224.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
CDO27_RS21255	558267..558686	+	420	WP_013845223.1	type II toxin-antitoxin system VapC family toxin	-
CDO27_RS21260	558694..558903	+	210	Protein_545	transposase	-
CDO27_RS21265	559058..559318	+	261	WP_013845222.1	ribbon-helix-helix domain-containing protein	Antitoxin
CDO27_RS21270	559318..559593	+	276	WP_014989489.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO27_RS21275	559621..560796	+	1176	WP_013845220.1	site-specific integrase	-
CDO27_RS21280	560845..561144	-	300	WP_013845219.1	hypothetical protein	-
CDO27_RS21290	561647..562084	-	438	WP_013845218.1	VOC family protein	-
CDO27_RS21300	562797..563102	+	306	Protein_551	hypothetical protein	-
CDO27_RS21305	563258..564205	-	948	WP_014989488.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIc	htpB	1..1549593	1549593
	-	flank	IS/Tn	-	-	558676..558903	227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10895.46 Da
• Isoelectric Point: 9.9707

>T77486 WP_014989489.1 NZ_CP021809:559318-559593 [Sinorhizobium meliloti]
MKLEWTSKAVADLGRLYDFLAPVNRQAAARTVQSWTSAPARLLEQPRIGERLEEFDPREVRRILVGHYEIRYEIRQSTIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T77486 NZ_CP021809:559318-559593 [Sinorhizobium meliloti]
ATGAAGCTTGAGTGGACGAGTAAGGCAGTCGCCGATCTTGGGCGCCTCTATGATTTCCTGGCACCGGTAAATCGGCAAGC
TGCGGCTCGTACTGTGCAATCATGGACGAGCGCTCCAGCACGCCTGCTCGAACAACCGCGCATCGGCGAACGGCTGGAGG
AATTCGACCCTCGAGAGGTTCGGCGAATTCTCGTTGGCCATTACGAAATACGATATGAGATCCGGCAGTCGACGATCTAC
GTGCTGCGGCTTTGGCATACGCGTGAAGACCGTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9652.01 Da
• Isoelectric Point: 4.8182

>AT77486 WP_013845222.1 NZ_CP021809:559058-559318 [Sinorhizobium meliloti]
METKVLTAHVPLPLAEKVDQIAARLERSRGWIVKQALTAWVDQEEERRRLTLEALADVDAGRVIDHQAVQAWADSLDSDK
PLSLPL

Nucleotide

• Length: 261 bp

>AT77486 NZ_CP021809:559058-559318 [Sinorhizobium meliloti]
ATGGAAACGAAGGTGCTGACGGCGCACGTACCACTGCCGCTCGCCGAGAAAGTCGATCAGATCGCGGCCCGGCTTGAGCG
CTCGCGCGGGTGGATCGTCAAGCAGGCGCTGACCGCCTGGGTCGATCAGGAAGAGGAGCGCCGACGCTTGACGCTGGAAG
CGCTTGCCGACGTCGACGCTGGCCGCGTTATCGATCACCAGGCCGTCCAGGCCTGGGCCGACAGTCTTGATAGTGACAAG
CCGCTCTCCTTGCCGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N8JT21