Detailed information of TA system TA077485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 249518..250116
• Replicon: plasmid psymA
• Accession: NZ_CP021809
• Organism: Sinorhizobium meliloti strain Rm41

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q930F0
• Locus tag: CDO27_RS19615
• Protein ID: WP_010967243.1
• Coordinates: 249518..249811 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CDO27_RS19620
• Protein ID: WP_014989717.1
• Coordinates: 249808..250116 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO27_RS19600	245742..246518	+	777	WP_014989720.1	histidinol-phosphatase	-
CDO27_RS19605	246896..247663	-	768	Protein_235	ParB N-terminal domain-containing protein	-
CDO27_RS19610	248057..249256	+	1200	WP_014989718.1	NAD-dependent formate dehydrogenase	-
CDO27_RS19615	249518..249811	-	294	WP_010967243.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
CDO27_RS19620	249808..250116	-	309	WP_014989717.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CDO27_RS19625	250326..251936	-	1611	WP_014989716.1	GMC family oxidoreductase N-terminal domain-containing protein	-
CDO27_RS19630	251933..253342	-	1410	WP_014531229.1	FAD-binding oxidoreductase	-
CDO27_RS19635	253342..254157	-	816	WP_014531228.1	shikimate dehydrogenase	-
CDO27_RS19640	254295..255107	-	813	WP_014531227.1	3-oxoacyl-ACP reductase FabG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIc	htpB	1..1549593	1549593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11278.93 Da
• Isoelectric Point: 6.4734

>T77485 WP_010967243.1 NZ_CP021809:c249811-249518 [Sinorhizobium meliloti]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAGTRELVIPRTPYIAAYMVMEDRIRI
LRVLHGAQKWPSELDDG

Nucleotide

• Length: 294 bp

>T77485 NZ_CP021809:c249811-249518 [Sinorhizobium meliloti]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGGAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11228.61 Da
• Isoelectric Point: 5.6604

>AT77485 WP_014989717.1 NZ_CP021809:c250116-249808 [Sinorhizobium meliloti]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAAMRKAAAGDR

Nucleotide

• Length: 309 bp

>AT77485 NZ_CP021809:c250116-249808 [Sinorhizobium meliloti]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTATGCGCAAGGCTGCGGCGGGTGATCGATGA

Structures

Toxin

• AlphaFold DB: Q930F0