Detailed information of TA system TA077484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 147024..147604
• Replicon: plasmid psymA
• Accession: NZ_CP021809
• Organism: Sinorhizobium meliloti strain Rm41

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO27_RS19085
• Protein ID: WP_080599828.1
• Coordinates: 147221..147604 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XCL0
• Locus tag: CDO27_RS19080
• Protein ID: WP_013851145.1
• Coordinates: 147024..147224 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO27_RS19055	142145..143251	+	1107	WP_014989789.1	FAD-binding oxidoreductase	-
CDO27_RS19060	143241..143555	+	315	WP_014528248.1	(2Fe-2S)-binding protein	-
CDO27_RS19065	143552..144928	+	1377	WP_014989788.1	NAD(P)/FAD-dependent oxidoreductase	-
CDO27_RS19070	145017..145790	-	774	WP_014989787.1	UTRA domain-containing protein	-
CDO27_RS19075	145981..146508	-	528	WP_080590796.1	TIGR02444 family protein	-
CDO27_RS19080	147024..147224	+	201	WP_013851145.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CDO27_RS19085	147221..147604	+	384	WP_080599828.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO27_RS36700	147759..148532	-	774	WP_172643728.1	RepB plasmid partition	-
CDO27_RS19095	148926..150125	+	1200	WP_014989784.1	NAD-dependent formate dehydrogenase	-
CDO27_RS19100	150621..151781	+	1161	WP_014989783.1	ImmA/IrrE family metallo-endopeptidase	-
CDO27_RS19105	151778..152272	+	495	WP_014989782.1	DUF4411 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIc	htpB	1..1549593	1549593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14256.47 Da
• Isoelectric Point: 6.9172

>T77484 WP_080599828.1 NZ_CP021809:147221-147604 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMIMIDRHAIFSI
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Nucleotide

• Length: 384 bp

>T77484 NZ_CP021809:147221-147604 [Sinorhizobium meliloti]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATAATGATCGATCGCCACGCCATTTTTAGCATC
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7096.22 Da
• Isoelectric Point: 11.1107

>AT77484 WP_013851145.1 NZ_CP021809:147024-147224 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT77484 NZ_CP021809:147024-147224 [Sinorhizobium meliloti]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7W6MID2