Detailed information of TA system TA077477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 975477..976131
• Replicon: chromosome
• Accession: NZ_CP021808
• Organism: Sinorhizobium meliloti strain Rm41

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: CDO27_RS04965
• Protein ID: WP_003530010.1
• Coordinates: 975477..975665 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: CDO27_RS04970
• Protein ID: WP_010969659.1
• Coordinates: 975670..976131 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO27_RS04950	972043..973353	+	1311	WP_010969656.1	HlyD family type I secretion periplasmic adaptor subunit	-
CDO27_RS04955	973378..974349	-	972	WP_017265202.1	glycosyltransferase family 2 protein	-
CDO27_RS04960	974530..975300	-	771	WP_010969658.1	hypothetical protein	-
CDO27_RS04965	975477..975665	+	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
CDO27_RS04970	975670..976131	+	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CDO27_RS04980	976990..977196	+	207	WP_015007715.1	hypothetical protein	-
CDO27_RS04985	977451..977723	+	273	WP_015007716.1	hypothetical protein	-
CDO27_RS04990	977734..978042	-	309	WP_015007717.1	hypothetical protein	-
CDO27_RS04995	978154..979128	-	975	WP_041169323.1	non-homologous end-joining DNA ligase	-
CDO27_RS36605	979221..979391	+	171	WP_015007719.1	hypothetical protein	-
CDO27_RS05010	979896..980069	-	174	WP_015007721.1	DUF3606 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	969369..1052155	82786

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T77477 WP_003530010.1 NZ_CP021808:975477-975665 [Sinorhizobium meliloti]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T77477 NZ_CP021808:975477-975665 [Sinorhizobium meliloti]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT77477 WP_010969659.1 NZ_CP021808:975670-976131 [Sinorhizobium meliloti]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT77477 NZ_CP021808:975670-976131 [Sinorhizobium meliloti]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA

Structures

Toxin

• AlphaFold DB: H0G1A3

Antitoxin

• AlphaFold DB: Q92NX0