Detailed information of TA system TA077474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1019725..1020410
• Replicon: plasmid psymA
• Accession: NZ_CP021805
• Organism: Sinorhizobium meliloti strain T073

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO28_RS23975
• Protein ID: WP_088196197.1
• Coordinates: 1019725..1020144 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CDO28_RS23980
• Protein ID: WP_088196198.1
• Coordinates: 1020141..1020410 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO28_RS23970	1019537..1019728	-	192	WP_088196196.1	hypothetical protein	-
CDO28_RS23975	1019725..1020144	-	420	WP_088196197.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO28_RS23980	1020141..1020410	-	270	WP_088196198.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDO28_RS23985	1020548..1021014	+	467	Protein_937	LysR family transcriptional regulator	-
CDO28_RS23990	1021104..1021979	-	876	WP_164831276.1	LysR family transcriptional regulator	-
CDO28_RS24000	1022464..1022649	+	186	Protein_939	hypothetical protein	-
CDO28_RS24005	1022706..1023086	-	381	WP_027992223.1	transcriptional regulator	-
CDO28_RS24010	1023104..1023415	-	312	WP_003526630.1	type II toxin-antitoxin system HigB family toxin	-
CDO28_RS24015	1023587..1024510	-	924	Protein_942	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1423499	1423499

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14703.93 Da
• Isoelectric Point: 4.8722

>T77474 WP_088196197.1 NZ_CP021805:c1020144-1019725 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSIQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTSSG

Nucleotide

• Length: 420 bp

>T77474 NZ_CP021805:c1020144-1019725 [Sinorhizobium meliloti]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTGCTGGCGCAGATCGAGACCATTCTTGGAAGTATACAAGTGCTTGCGCTCGATGTGCCAGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTTCTTCGGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10202.68 Da
• Isoelectric Point: 4.5716

>AT77474 WP_088196198.1 NZ_CP021805:c1020410-1020141 [Sinorhizobium meliloti]
MPQLRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT77474 NZ_CP021805:c1020410-1020141 [Sinorhizobium meliloti]
ATGCCCCAGCTCCGACACAACGAACCAACACCGCCAAGGGAAGCCAAGCTCTTCCGCAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCGGGCGATCGAGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAAGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCTGACGTTGACGAAACC
CTGCTGCCGGTGAAGGAAATCGACCTGTGA