Detailed information of TA system TA077472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 419506..420176
• Replicon: plasmid psymA
• Accession: NZ_CP021805
• Organism: Sinorhizobium meliloti strain T073

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO28_RS20990
• Protein ID: WP_015242680.1
• Coordinates: 419757..420176 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6A7ZM99
• Locus tag: CDO28_RS20985
• Protein ID: WP_015242681.1
• Coordinates: 419506..419760 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO28_RS20965	415681..416616	+	936	WP_010967052.1	ABC transporter permease	-
CDO28_RS20970	416622..417443	+	822	WP_088195919.1	ABC transporter permease	-
CDO28_RS20975	417440..418354	+	915	WP_088195920.1	ABC transporter ATP-binding protein	-
CDO28_RS20980	418351..419313	+	963	WP_015242682.1	ABC transporter ATP-binding protein	-
CDO28_RS20985	419506..419760	+	255	WP_015242681.1	plasmid stability protein	Antitoxin
CDO28_RS20990	419757..420176	+	420	WP_015242680.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO28_RS20995	420145..420573	-	429	WP_153404381.1	GFA family protein	-
CDO28_RS21000	420975..423509	+	2535	WP_041169808.1	PAS domain S-box protein	-
CDO28_RS21005	423537..423899	+	363	WP_015242677.1	response regulator	-
CDO28_RS21010	423956..425116	-	1161	WP_015242676.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1423499	1423499

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14761.01 Da
• Isoelectric Point: 6.7266

>T77472 WP_015242680.1 NZ_CP021805:419757-420176 [Sinorhizobium meliloti]
MIVLGTNVVSEAMKPTPDPAVRSWLNEQVAETLYLASVTLAELLFGIGALPDGRRKKALTEMLGGVLELFGDRVLPFDIG
AARHYAELAVTARAAGKGFPTADGYIAAIAASRGFIVATRDSSPFEAAGLTVVNPWHHR

Nucleotide

• Length: 420 bp

>T77472 NZ_CP021805:419757-420176 [Sinorhizobium meliloti]
ATGATTGTCCTCGGTACGAATGTCGTCTCCGAGGCGATGAAACCGACCCCCGATCCGGCCGTGCGCTCCTGGTTGAACGA
GCAGGTCGCTGAAACCTTGTATCTGGCCAGTGTGACCTTGGCCGAACTGCTGTTCGGCATCGGTGCGCTGCCGGACGGCC
GGCGCAAGAAGGCTCTGACCGAAATGCTTGGCGGCGTGCTCGAACTGTTTGGCGATCGCGTATTGCCGTTCGACATCGGA
GCTGCTCGCCATTATGCCGAGCTTGCGGTGACCGCTCGAGCGGCCGGCAAGGGATTTCCGACGGCCGACGGATATATTGC
CGCCATTGCCGCCTCCAGGGGATTTATCGTCGCGACCCGCGATAGCAGCCCGTTCGAAGCGGCTGGACTTACCGTGGTAA
ATCCTTGGCACCATCGATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9480.82 Da
• Isoelectric Point: 6.4732

>AT77472 WP_015242681.1 NZ_CP021805:419506-419760 [Sinorhizobium meliloti]
MAILTVRNVPDEVHRALRVRAAMHGRSTEAEVREILESAVKPEQRVRMGDALAELGRRLGLTNDDFAVFDEVRDKAPAQP
VRFE

Nucleotide

• Length: 255 bp

>AT77472 NZ_CP021805:419506-419760 [Sinorhizobium meliloti]
ATGGCGATCCTGACGGTCCGGAATGTGCCCGATGAAGTGCATCGCGCCTTGCGCGTGAGAGCGGCGATGCACGGCCGCAG
CACCGAGGCGGAAGTCCGGGAGATTTTGGAAAGTGCGGTGAAGCCCGAGCAGCGCGTTCGCATGGGCGATGCCCTGGCCG
AACTCGGCCGGCGACTGGGGCTGACCAATGACGATTTCGCCGTGTTCGACGAGGTACGCGACAAGGCGCCGGCGCAGCCC
GTGAGATTTGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6A7ZM99