Detailed information of TA system TA077468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 2841575..2842341
• Replicon: chromosome
• Accession: NZ_CP021804
• Organism: Sinorhizobium meliloti strain T073

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: CDO28_RS13970
• Protein ID: WP_027990219.1
• Coordinates: 2841575..2842057 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: H0G6D4
• Locus tag: CDO28_RS13975
• Protein ID: WP_003533490.1
• Coordinates: 2842057..2842341 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO28_RS13940	2837316..2838191	+	876	WP_010970093.1	YihY/virulence factor BrkB family protein	-
CDO28_RS13945	2838210..2839106	-	897	WP_027990160.1	tRNA glutamyl-Q(34) synthetase GluQRS	-
CDO28_RS13950	2839259..2839906	+	648	WP_027993538.1	DNA-3-methyladenine glycosylase	-
CDO28_RS13955	2840012..2840569	+	558	WP_003533486.1	HNH endonuclease	-
CDO28_RS13960	2840601..2841110	-	510	WP_003533487.1	disulfide bond formation protein B	-
CDO28_RS13970	2841575..2842057	-	483	WP_027990219.1	GNAT family N-acetyltransferase	Toxin
CDO28_RS13975	2842057..2842341	-	285	WP_003533490.1	DUF1778 domain-containing protein	Antitoxin
CDO28_RS13980	2842546..2844192	+	1647	WP_088195577.1	AMP-binding protein	-
CDO28_RS13985	2844208..2845479	-	1272	WP_010970087.1	GNAT family N-acetyltransferase	-
CDO28_RS13990	2845483..2846871	-	1389	WP_003533497.1	lipopolysaccharide biosynthesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17419.87 Da
• Isoelectric Point: 7.9232

>T77468 WP_027990219.1 NZ_CP021804:c2842057-2841575 [Sinorhizobium meliloti]
MLSAPTPLGEAHDFDLFQSGNATLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Nucleotide

• Length: 483 bp

>T77468 NZ_CP021804:c2842057-2841575 [Sinorhizobium meliloti]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGCCACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10771.12 Da
• Isoelectric Point: 7.3175

>AT77468 WP_003533490.1 NZ_CP021804:c2842341-2842057 [Sinorhizobium meliloti]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Nucleotide

• Length: 285 bp

>AT77468 NZ_CP021804:c2842341-2842057 [Sinorhizobium meliloti]
ATGGCCACGAAATCGTCGACTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Structures

Antitoxin

• AlphaFold DB: H0G6D4