Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2157489..2158150 Replicon chromosome
Accession NZ_CP021804
Organism Sinorhizobium meliloti strain T073

Toxin (Protein)


Gene name vapC Uniprot ID Q92KY5
Locus tag CDO28_RS10790 Protein ID WP_010970565.1
Coordinates 2157746..2158150 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q92KY4
Locus tag CDO28_RS10785 Protein ID WP_010970566.1
Coordinates 2157489..2157749 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO28_RS10755 2152963..2153844 + 882 WP_010970570.1 pyridoxal kinase PdxY -
CDO28_RS10760 2154018..2154701 + 684 WP_010970569.1 carbonic anhydrase -
CDO28_RS10770 2155048..2155869 - 822 WP_010970568.1 lytic transglycosylase family protein -
CDO28_RS10775 2156042..2157076 - 1035 WP_003531790.1 aspartate-semialdehyde dehydrogenase -
CDO28_RS10780 2157190..2157360 - 171 WP_010970567.1 hypothetical protein -
CDO28_RS10785 2157489..2157749 + 261 WP_010970566.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CDO28_RS10790 2157746..2158150 + 405 WP_010970565.1 type II toxin-antitoxin system VapC family toxin Toxin
CDO28_RS10795 2158669..2160558 + 1890 WP_014527006.1 MHS family MFS transporter -
CDO28_RS10800 2160659..2161366 - 708 WP_010970563.1 phosphatase PAP2 family protein -
CDO28_RS10805 2161495..2162607 - 1113 WP_003535893.1 3-isopropylmalate dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(8-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14507.71 Da        Isoelectric Point: 6.7257

>T77466 WP_010970565.1 NZ_CP021804:2157746-2158150 [Sinorhizobium meliloti]
VISHILDTNAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Download         Length: 405 bp

>T77466 NZ_CP021804:2157746-2158150 [Sinorhizobium meliloti]
GTGATCTCCCATATTCTCGACACCAACGCCGTCATCGCTTTGATCGGGCGCAAATCGGATGCATTGGTCACCCGTGTACT
CCATAGCCCTCAGGGCATTATCGGGCTCCCGTCGGTCGTCGCGTATGAACTCTATTTTGGAGCGCAGAAAAGCGCGAAGG
CCCAACACAATCTCGAAACCCTGCGTTTGTTGATGGCGGATTTTCCGATCCTCGACTTCGACCGGAACGATGCCTTTGTC
GCAGGCGAGATCCGGGCCGCCCTTGCCGCGAAGGGTACGCCCATCGGACCTTATGACGTGCTGATTGCGGGACAGGCCAA
AGCCCGTGGATTGACGCTCGTCACCAACAATGTCGGCGAGTTCAATAGGGTTGAGAACCTGCGCGTGGAGGATTGGTCGC
TCTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9903.16 Da        Isoelectric Point: 5.1253

>AT77466 WP_010970566.1 NZ_CP021804:2157489-2157749 [Sinorhizobium meliloti]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRSE
LDAVFR

Download         Length: 261 bp

>AT77466 NZ_CP021804:2157489-2157749 [Sinorhizobium meliloti]
ATGCCCCATCTTGCACGGGTGTTCCAGTCCGGAAATTCGCAGGCCGTCCGCCTGCCTAAGGAATTCCGCTTCAACGTCGA
TCGCGTCGAAATCACGCAGGAAGGCGACGCGCTCATTCTCCGCCCGCACGTCGAGCAAGGCGAGCAGTGGTCATCGCTGA
AGGCGGCACTTGCCCGTGGCATGAGTGAAGACTTCATGATGTGCGGGCGCGAGCAGCCGGAACAGCAGGATCGGTCGGAA
CTCGACGCGGTATTTCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92KY5

Antitoxin

Source ID Structure
AlphaFold DB Q92KY4

References