Detailed information of TA system TA077465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1475570..1476147
• Replicon: chromosome
• Accession: NZ_CP021804
• Organism: Sinorhizobium meliloti strain T073

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q92S20
• Locus tag: CDO28_RS07570
• Protein ID: WP_010968707.1
• Coordinates: 1475884..1476147 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: CDO28_RS07565
• Protein ID: WP_010968708.1
• Coordinates: 1475570..1475887 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO28_RS07535	1471046..1471435	+	390	WP_013844021.1	DUF930 domain-containing protein	-
CDO28_RS07540	1471715..1472026	-	312	WP_004435964.1	hypothetical protein	-
CDO28_RS07545	1472229..1473311	-	1083	WP_010968711.1	nucleotidyltransferase family protein	-
CDO28_RS07550	1473298..1474398	-	1101	WP_014526604.1	HPr kinase	-
CDO28_RS07555	1474718..1474906	+	189	WP_013844020.1	hypothetical protein	-
CDO28_RS07560	1474903..1475367	+	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
CDO28_RS07565	1475570..1475887	-	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
CDO28_RS07570	1475884..1476147	-	264	WP_010968707.1	BrnT family toxin	Toxin
CDO28_RS07580	1476732..1476944	-	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
CDO28_RS07585	1476949..1477569	-	621	WP_004435951.1	Maf-like protein	-
CDO28_RS07590	1477667..1477885	-	219	WP_004435948.1	translation initiation factor IF-1	-
CDO28_RS07595	1478043..1478522	-	480	WP_010968705.1	low molecular weight phosphatase family protein	-
CDO28_RS07600	1478536..1479012	-	477	WP_010968704.1	UPF0262 family protein	-
CDO28_RS07605	1479009..1480313	-	1305	WP_010968703.1	histidinol dehydrogenase	-
CDO28_RS07610	1480378..1480818	-	441	WP_010968702.1	DUF2948 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T77465 WP_010968707.1 NZ_CP021804:c1476147-1475884 [Sinorhizobium meliloti]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASALIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T77465 NZ_CP021804:c1476147-1475884 [Sinorhizobium meliloti]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCCTCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT77465 WP_010968708.1 NZ_CP021804:c1475887-1475570 [Sinorhizobium meliloti]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT77465 NZ_CP021804:c1475887-1475570 [Sinorhizobium meliloti]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCAAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Structures

Toxin

• AlphaFold DB: Q92S20

Antitoxin

• AlphaFold DB: Q92KN5