Detailed information of TA system TA077461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1913068..1913735
• Replicon: plasmid psymB
• Accession: NZ_CP021802
• Organism: Sinorhizobium meliloti strain USDA1021

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO29_RS34035
• Protein ID: WP_013849979.1
• Coordinates: 1913292..1913735 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92WE1
• Locus tag: CDO29_RS34030
• Protein ID: WP_010975287.1
• Coordinates: 1913068..1913295 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO29_RS34005	1908461..1909342	-	882	WP_003525869.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
CDO29_RS34010	1909460..1910752	-	1293	WP_013849976.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
CDO29_RS34020	1911122..1912411	+	1290	WP_015008544.1	SDR family oxidoreductase	-
CDO29_RS34025	1912411..1912884	+	474	WP_015008545.1	DUF2269 family protein	-
CDO29_RS34030	1913068..1913295	+	228	WP_010975287.1	hypothetical protein	Antitoxin
CDO29_RS34035	1913292..1913735	+	444	WP_013849979.1	PIN domain-containing protein	Toxin
CDO29_RS34040	1913751..1914689	-	939	WP_088203966.1	choline ABC transporter substrate-binding protein	-
CDO29_RS34045	1914758..1915588	-	831	WP_013849981.1	SDR family oxidoreductase	-
CDO29_RS34050	1915585..1916082	-	498	WP_015008548.1	acetone carboxylase subunit gamma	-
CDO29_RS34055	1916086..1918278	-	2193	WP_015008549.1	hydantoinase B/oxoprolinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	fliQ / flhA / icl / htpB / htpB	1..2011522	2011522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15650.94 Da
• Isoelectric Point: 6.6378

>T77461 WP_013849979.1 NZ_CP021802:1913292-1913735 [Sinorhizobium meliloti]
VTFLLDVNVLIALIDPSHIGHDDAHEWFASIGQTTWATCPITENGVIRIVGNPEYPNSPGSPLPVMEIVAKLRSLPGHVF
WPDDVSLVGSSDIIPSKILTSGQVTDTYLLALAKARGGKLATFDRKLSAAAVTKGNSALHLIATNRS

Nucleotide

• Length: 444 bp

>T77461 NZ_CP021802:1913292-1913735 [Sinorhizobium meliloti]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGAGCCATATAGGCCATGATGATGCGCACGAATG
GTTCGCGTCGATTGGTCAGACCACGTGGGCCACCTGCCCAATTACCGAAAATGGTGTCATCCGGATCGTCGGCAATCCCG
AATATCCCAATTCTCCCGGCTCACCGTTACCCGTGATGGAGATCGTCGCCAAATTGCGGTCGCTCCCCGGTCATGTTTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCAAGTGATATCATCCCATCGAAAATCCTGACTTCGGGGCAGGTGACTGACAC
CTATCTGCTTGCGCTCGCCAAGGCGCGTGGGGGCAAGCTGGCGACATTCGATCGCAAGCTGTCGGCAGCGGCGGTCACGA
AGGGCAATTCCGCCTTGCACCTGATCGCGACGAATAGGTCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8174.39 Da
• Isoelectric Point: 9.1096

>AT77461 WP_010975287.1 NZ_CP021802:1913068-1913295 [Sinorhizobium meliloti]
MRTTLAIDDDVLIAAKAMATQQQRSVGEVISELARRSLRRPRSGGERNGIPLLSPRPDAPPVTLEIVNALRDELA

Nucleotide

• Length: 228 bp

>AT77461 NZ_CP021802:1913068-1913295 [Sinorhizobium meliloti]
ATGAGAACGACCTTGGCTATTGACGACGATGTCCTGATCGCCGCAAAGGCAATGGCGACCCAACAGCAGCGGAGCGTCGG
CGAGGTAATTTCCGAACTGGCGAGGCGTTCTCTGCGCCGGCCGCGCAGCGGCGGCGAGCGTAATGGAATTCCTCTCCTGT
CGCCTCGTCCGGACGCACCACCCGTGACGCTCGAGATCGTCAACGCCCTGCGTGACGAACTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: Q92WE1