Detailed information of TA system TA077460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 924551..925085
• Replicon: plasmid psymB
• Accession: NZ_CP021802
• Organism: Sinorhizobium meliloti strain USDA1021

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CDO29_RS29190
• Protein ID: WP_088203768.1
• Coordinates: 924789..925085 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: CDO29_RS29185
• Protein ID: WP_003527850.1
• Coordinates: 924551..924799 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO29_RS29165	920781..922112	+	1332	WP_088203767.1	O-antigen ligase family protein	-
CDO29_RS29170	922105..922860	+	756	WP_017270253.1	polysaccharide deacetylase family protein	-
CDO29_RS29175	922871..924100	+	1230	WP_027988592.1	glycosyltransferase family 4 protein	-
CDO29_RS29180	924217..924465	+	249	WP_164830233.1	hypothetical protein	-
CDO29_RS29185	924551..924799	+	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CDO29_RS29190	924789..925085	+	297	WP_088203768.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO29_RS29195	925163..925426	-	264	Protein_884	helix-turn-helix domain containing protein	-
CDO29_RS29200	925748..926917	+	1170	WP_017276026.1	glycosyltransferase family 4 protein	-
CDO29_RS29205	926914..927303	+	390	WP_026030012.1	hypothetical protein	-
CDO29_RS29210	927334..928377	+	1044	WP_027988589.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	fliQ / flhA / icl / htpB / htpB	1..2011522	2011522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-93)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11474.20 Da
• Isoelectric Point: 10.7543

>T77460 WP_088203768.1 NZ_CP021802:924789-925085 [Sinorhizobium meliloti]
MATERPFRLAPAAKADLRKIWRYKARRWSLEQAETYQDQLYTAFEGLAAGTKKGRNVDVRPGYLKYPAGAHIVYFRDRGD
RIDIIRILHGRMDAQRHL

Nucleotide

• Length: 297 bp

>T77460 NZ_CP021802:924789-925085 [Sinorhizobium meliloti]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTGCCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACAAGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGCAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGGATGGACGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT77460 WP_003527850.1 NZ_CP021802:924551-924799 [Sinorhizobium meliloti]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT77460 NZ_CP021802:924551-924799 [Sinorhizobium meliloti]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CTTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Antitoxin

• AlphaFold DB: H0FXK0