Detailed information of TA system TA077458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 534179..534756
• Replicon: plasmid psymB
• Accession: NZ_CP021802
• Organism: Sinorhizobium meliloti strain USDA1021

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: CDO29_RS27210
• Protein ID: WP_014528972.1
• Coordinates: 534179..534442 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: CDO29_RS27215
• Protein ID: WP_010968708.1
• Coordinates: 534439..534756 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO29_RS27170	529508..529948	+	441	WP_014528946.1	DUF2948 family protein	-
CDO29_RS27175	530012..531316	+	1305	WP_028004761.1	histidinol dehydrogenase	-
CDO29_RS27180	531313..531789	+	477	WP_010968704.1	UPF0262 family protein	-
CDO29_RS27185	531803..532282	+	480	WP_010968705.1	low molecular weight phosphatase family protein	-
CDO29_RS27190	532440..532658	+	219	WP_004435948.1	translation initiation factor IF-1	-
CDO29_RS27195	532757..533377	+	621	WP_014528949.1	Maf-like protein	-
CDO29_RS27200	533382..533594	+	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
CDO29_RS27210	534179..534442	+	264	WP_014528972.1	BrnT family toxin	Toxin
CDO29_RS27215	534439..534756	+	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
CDO29_RS27220	534959..535423	-	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
CDO29_RS27225	535420..535608	-	189	WP_017266533.1	hypothetical protein	-
CDO29_RS27230	535927..537027	+	1101	WP_088203729.1	serine kinase	-
CDO29_RS27235	537014..538096	+	1083	WP_014528975.1	nucleotidyltransferase family protein	-
CDO29_RS27240	538299..538610	+	312	WP_014528976.1	hypothetical protein	-
CDO29_RS27245	538889..539278	-	390	WP_013844021.1	DUF930 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	fliQ / flhA / icl / htpB / htpB	1..2011522	2011522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T77458 WP_014528972.1 NZ_CP021802:534179-534442 [Sinorhizobium meliloti]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASAIIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T77458 NZ_CP021802:534179-534442 [Sinorhizobium meliloti]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCATCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT77458 WP_010968708.1 NZ_CP021802:534439-534756 [Sinorhizobium meliloti]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT77458 NZ_CP021802:534439-534756 [Sinorhizobium meliloti]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCAAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Structures

Antitoxin

• AlphaFold DB: Q92KN5