Detailed information of TA system TA077457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 251769..252303
• Replicon: plasmid psymB
• Accession: NZ_CP021802
• Organism: Sinorhizobium meliloti strain USDA1021

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q92X99
• Locus tag: CDO29_RS25900
• Protein ID: WP_010974986.1
• Coordinates: 251769..252065 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: CDO29_RS25905
• Protein ID: WP_003527850.1
• Coordinates: 252055..252303 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO29_RS25870	247213..248616	-	1404	WP_010974991.1	sodium:alanine symporter family protein	-
CDO29_RS25875	249150..249359	-	210	WP_003527838.1	dodecin family protein	-
CDO29_RS25880	249649..250095	+	447	WP_041170054.1	DNA-binding protein	-
CDO29_RS25885	250099..250284	-	186	WP_010974989.1	hypothetical protein	-
CDO29_RS25890	250451..250849	-	399	WP_010974988.1	hypothetical protein	-
CDO29_RS25895	251428..251688	+	261	Protein_246	helix-turn-helix domain containing protein	-
CDO29_RS25900	251769..252065	-	297	WP_010974986.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO29_RS25905	252055..252303	-	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CDO29_RS25910	252411..252566	-	156	WP_010974985.1	hypothetical protein	-
CDO29_RS25920	252922..253047	+	126	Protein_250	IS481 family transposase	-
CDO29_RS25925	253206..254402	-	1197	WP_088203703.1	IS256 family transposase	-
CDO29_RS25930	254494..255273	-	780	WP_010974982.1	methyltransferase domain-containing protein	-
CDO29_RS25935	255277..256077	-	801	WP_010974981.1	ABC transporter ATP-binding protein	-
CDO29_RS25940	256074..257117	-	1044	WP_088203704.1	iron ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	fliQ / flhA / icl / htpB / htpB	1..2011522	2011522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-91)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11473.15 Da
• Isoelectric Point: 10.6593

>T77457 WP_010974986.1 NZ_CP021802:c252065-251769 [Sinorhizobium meliloti]
MATERPFRLAPTAKADLRKIWRYTARRWSLEQAETYQDQLYTAFEGLAVGTKKGRNVDVRPGYLKYPAGAHIVYFRDRGD
RIDIIRILHGRVDAQRHL

Nucleotide

• Length: 297 bp

>T77457 NZ_CP021802:c252065-251769 [Sinorhizobium meliloti]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTACCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGTAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGTGTGGACGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT77457 WP_003527850.1 NZ_CP021802:c252303-252055 [Sinorhizobium meliloti]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT77457 NZ_CP021802:c252303-252055 [Sinorhizobium meliloti]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CTTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Toxin

• AlphaFold DB: Q92X99

Antitoxin

• AlphaFold DB: H0FXK0