Detailed information of TA system TA077456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 863473..864158
• Replicon: plasmid psymA
• Accession: NZ_CP021801
• Organism: Sinorhizobium meliloti strain USDA1021

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO29_RS21740
• Protein ID: WP_017263896.1
• Coordinates: 863739..864158 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XET8
• Locus tag: CDO29_RS21735
• Protein ID: WP_013845417.1
• Coordinates: 863473..863742 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO29_RS21705	858991..859860	-	870	WP_088203554.1	aldo/keto reductase family oxidoreductase	-
CDO29_RS21710	859977..860899	+	923	Protein_841	LysR family transcriptional regulator	-
CDO29_RS21725	861879..862754	+	876	WP_164819965.1	LysR family transcriptional regulator	-
CDO29_RS21730	862865..863335	-	471	Protein_843	LysR family transcriptional regulator	-
CDO29_RS21735	863473..863742	+	270	WP_013845417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDO29_RS21740	863739..864158	+	420	WP_017263896.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO29_RS21750	864821..865132	+	312	WP_026031097.1	type II toxin-antitoxin system HigB family toxin	-
CDO29_RS21755	865150..865530	+	381	WP_003526626.1	transcriptional regulator	-
CDO29_RS21760	865800..867173	+	1374	WP_014528618.1	ISNCY family transposase	-
CDO29_RS21765	867532..868959	+	1428	WP_088203555.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..1412628	1412628

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.00 Da
• Isoelectric Point: 4.8722

>T77456 WP_017263896.1 NZ_CP021801:863739-864158 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSG

Nucleotide

• Length: 420 bp

>T77456 NZ_CP021801:863739-864158 [Sinorhizobium meliloti]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTACTGGCGCAGATCGAGACCATTCTTGGAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTCCTTCGGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10203.67 Da
• Isoelectric Point: 4.4798

>AT77456 WP_013845417.1 NZ_CP021801:863473-863742 [Sinorhizobium meliloti]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT77456 NZ_CP021801:863473-863742 [Sinorhizobium meliloti]
ATGCCCGAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCTAAGCTCTTCCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAGGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCCGACGTTGACGAAACC
CTGCTGCCGGTGAAGGAAATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A844A3Y5