Detailed information of TA system TA077455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 518372..518871
• Replicon: plasmid psymA
• Accession: NZ_CP021801
• Organism: Sinorhizobium meliloti strain USDA1021

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q92YA6
• Locus tag: CDO29_RS19990
• Protein ID: WP_010967939.1
• Coordinates: 518698..518871 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CDO29_RS37030
• Protein ID: WP_017272263.1
• Coordinates: 518372..518701 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO29_RS19955	513747..513923	+	177	WP_013845643.1	hypothetical protein	-
CDO29_RS19960	514049..514942	-	894	WP_010967943.1	LysR family transcriptional regulator	-
CDO29_RS19965	515034..515477	+	444	WP_010967942.1	PACE efflux transporter	-
CDO29_RS19970	515594..515932	+	339	WP_013845641.1	SDR family oxidoreductase	-
CDO29_RS19975	515916..516257	-	342	Protein_514	hypothetical protein	-
CDO29_RS19980	516641..518011	-	1371	WP_017265792.1	alpha/beta hydrolase	-
CDO29_RS37030	518372..518701	+	330	WP_017272263.1	hypothetical protein	Antitoxin
CDO29_RS19990	518698..518871	+	174	WP_010967939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO29_RS19995	518969..519274	-	306	WP_013845638.1	hypothetical protein	-
CDO29_RS20000	519360..519599	-	240	WP_017272264.1	hypothetical protein	-
CDO29_RS20005	519592..519951	-	360	WP_013845635.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
CDO29_RS20010	520546..520797	+	252	WP_003534243.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
CDO29_RS20015	520825..521310	+	486	WP_014531392.1	YidB family protein	-
CDO29_RS20020	521680..522996	-	1317	WP_088203481.1	aspartate aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..1412628	1412628

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Antitoxin

• Toxin YoeB (5-84)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6497.32 Da
• Isoelectric Point: 5.7733

>T77455 WP_010967939.1 NZ_CP021801:518698-518871 [Sinorhizobium meliloti]
MKLVWARYALDDRDAIFSYIERENPRAAVHVDEEVVSAGRPLDFPESRRPGRIAGTP

Nucleotide

• Length: 174 bp

>T77455 NZ_CP021801:518698-518871 [Sinorhizobium meliloti]
ATGAAGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACGCCATCTTCAGCTACATCGAACGAGAGAATCCGAGGGC
GGCGGTCCACGTTGACGAGGAGGTCGTCAGCGCTGGTCGTCCGCTGGACTTTCCCGAAAGCCGCCGCCCTGGCCGGATTG
CCGGAACTCCATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12025.64 Da
• Isoelectric Point: 11.1401

>AT77455 WP_017272263.1 NZ_CP021801:518372-518701 [Sinorhizobium meliloti]
VSERLLLIALLALGPFRASQRHTGRPALLRRGPQSAAGAVEEHFIQLRAPLWLITLGGFSHREAHDAWFRAKALRALEDT
RPDADDADADAHFQERRAAALRKAAAGDR

Nucleotide

• Length: 330 bp

>AT77455 NZ_CP021801:518372-518701 [Sinorhizobium meliloti]
GTGAGCGAGCGCCTTCTGCTCATCGCCCTCCTGGCCCTCGGTCCGTTCCGAGCCAGCCAGCGGCATACCGGTCGTCCTGC
TCTGCTACGGCGTGGCCCCCAATCGGCCGCCGGCGCGGTCGAGGAGCACTTCATCCAACTCCGCGCTCCGCTGTGGCTGA
TCACGCTCGGCGGTTTCAGCCACAGAGAGGCTCACGACGCTTGGTTCCGCGCCAAAGCTTTGCGGGCACTCGAGGACACT
CGACCCGATGCGGACGACGCCGACGCCGATGCGCACTTTCAAGAGCGCCGCGCCGCAGCTTTGCGCAAGGCTGCGGCGGG
TGATCGATGA

Structures

Toxin

• AlphaFold DB: Q92YA6