Detailed information of TA system TA077454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 107761..108356
• Replicon: plasmid psymA
• Accession: NZ_CP021801
• Organism: Sinorhizobium meliloti strain USDA1021

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F7XBJ0
• Locus tag: CDO29_RS17730
• Protein ID: WP_014531932.1
• Coordinates: 108066..108356 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q930F1
• Locus tag: CDO29_RS17725
• Protein ID: WP_010967242.1
• Coordinates: 107761..108069 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO29_RS17705	103962..105746	+	1785	WP_014531184.1	polysaccharide biosynthesis/export family protein	-
CDO29_RS17715	106786..107052	+	267	WP_010967197.1	EAL domain-containing protein	-
CDO29_RS17720	107204..107416	-	213	WP_013845891.1	hypothetical protein	-
CDO29_RS17725	107761..108069	+	309	WP_010967242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CDO29_RS17730	108066..108356	+	291	WP_014531932.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
CDO29_RS17735	108618..109817	-	1200	WP_014531931.1	NAD-dependent formate dehydrogenase	-
CDO29_RS17740	110091..110348	+	258	WP_080567622.1	hypothetical protein	-
CDO29_RS17745	110440..111440	-	1001	Protein_117	IS30 family transposase	-
CDO29_RS17750	111882..112781	-	900	WP_010967198.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..1412628	1412628
	-	flank	IS/Tn	-	-	110440..110814	374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11195.84 Da
• Isoelectric Point: 6.4734

>T77454 WP_014531932.1 NZ_CP021801:108066-108356 [Sinorhizobium meliloti]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAATRELVIPRTYIAAYMVMEDRIRIL
RVLHGAQKWPSELDDG

Nucleotide

• Length: 291 bp

>T77454 NZ_CP021801:108066-108356 [Sinorhizobium meliloti]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGCAACTCGAGAGCTTGTTATCCCTCGGACCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATTCTG
CGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11210.58 Da
• Isoelectric Point: 5.6604

>AT77454 WP_010967242.1 NZ_CP021801:107761-108069 [Sinorhizobium meliloti]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAALRKAAAGDR

Nucleotide

• Length: 309 bp

>AT77454 NZ_CP021801:107761-108069 [Sinorhizobium meliloti]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Structures

Toxin

• AlphaFold DB: F7XBJ0

Antitoxin

• AlphaFold DB: Q930F1