Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 65053..65633 Replicon plasmid psymA
Accession NZ_CP021801
Organism Sinorhizobium meliloti strain USDA1021

Toxin (Protein)


Gene name vapC Uniprot ID Q92XP1
Locus tag CDO29_RS17450 Protein ID WP_010968151.1
Coordinates 65053..65436 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID F7XCL0
Locus tag CDO29_RS17455 Protein ID WP_013851145.1
Coordinates 65433..65633 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO29_RS17415 60120..60608 + 489 WP_088203420.1 hypothetical protein -
CDO29_RS36485 60621..61355 + 735 WP_127570026.1 acyltransferase -
CDO29_RS17430 61945..62933 + 989 Protein_60 IS21 family transposase -
CDO29_RS17435 62930..63811 + 882 WP_088203423.1 IS21-like element helper ATPase IstB -
CDO29_RS17445 64203..64577 - 375 WP_088203424.1 LysE family transporter -
CDO29_RS17450 65053..65436 - 384 WP_010968151.1 type II toxin-antitoxin system VapC family toxin Toxin
CDO29_RS17455 65433..65633 - 201 WP_013851145.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CDO29_RS17460 65924..66325 + 402 WP_014531161.1 GFA family protein -
CDO29_RS17465 66402..67238 - 837 WP_014531162.1 helix-turn-helix transcriptional regulator -
CDO29_RS17470 67334..67657 + 324 WP_017266777.1 DUF1127 domain-containing protein -
CDO29_RS17475 67696..68424 + 729 WP_014531163.1 class I SAM-dependent methyltransferase -
CDO29_RS17480 68518..68664 + 147 WP_017266778.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - htpB 1..1412628 1412628
- flank IS/Tn - - 62930..63811 881


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14292.54 Da        Isoelectric Point: 6.9172

>T77453 WP_010968151.1 NZ_CP021801:c65436-65053 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Download         Length: 384 bp

>T77453 NZ_CP021801:c65436-65053 [Sinorhizobium meliloti]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7096.22 Da        Isoelectric Point: 11.1107

>AT77453 WP_013851145.1 NZ_CP021801:c65633-65433 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT77453 NZ_CP021801:c65633-65433 [Sinorhizobium meliloti]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92XP1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6MID2

References