Detailed information of TA system TA077451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 3047999..3048765
• Replicon: chromosome
• Accession: NZ_CP021800
• Organism: Sinorhizobium meliloti strain USDA1021

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: H0G6D3
• Locus tag: CDO29_RS15170
• Protein ID: WP_003533488.1
• Coordinates: 3047999..3048481 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: H0G6D4
• Locus tag: CDO29_RS15175
• Protein ID: WP_003533490.1
• Coordinates: 3048481..3048765 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO29_RS15140	3043740..3044615	+	876	WP_010970093.1	YihY/virulence factor BrkB family protein	-
CDO29_RS15145	3044634..3045530	-	897	WP_010970092.1	tRNA glutamyl-Q(34) synthetase GluQRS	-
CDO29_RS15150	3045683..3046330	+	648	WP_010970091.1	DNA-3-methyladenine glycosylase	-
CDO29_RS15155	3046436..3046993	+	558	WP_003533486.1	HNH endonuclease	-
CDO29_RS15160	3047025..3047534	-	510	WP_013844819.1	disulfide bond formation protein B	-
CDO29_RS15170	3047999..3048481	-	483	WP_003533488.1	GNAT family N-acetyltransferase	Toxin
CDO29_RS15175	3048481..3048765	-	285	WP_003533490.1	DUF1778 domain-containing protein	Antitoxin
CDO29_RS15180	3048970..3050616	+	1647	WP_088203365.1	AMP-binding protein	-
CDO29_RS15185	3050632..3051903	-	1272	WP_010970087.1	GNAT family N-acetyltransferase	-
CDO29_RS15190	3051907..3053295	-	1389	WP_003533497.1	lipopolysaccharide biosynthesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17463.88 Da
• Isoelectric Point: 7.3827

>T77451 WP_003533488.1 NZ_CP021800:c3048481-3047999 [Sinorhizobium meliloti]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Nucleotide

• Length: 483 bp

>T77451 NZ_CP021800:c3048481-3047999 [Sinorhizobium meliloti]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGACACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10771.12 Da
• Isoelectric Point: 7.3175

>AT77451 WP_003533490.1 NZ_CP021800:c3048765-3048481 [Sinorhizobium meliloti]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Nucleotide

• Length: 285 bp

>AT77451 NZ_CP021800:c3048765-3048481 [Sinorhizobium meliloti]
ATGGCCACGAAATCGTCGACTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Structures

Toxin

• AlphaFold DB: H0G6D3

Antitoxin

• AlphaFold DB: H0G6D4