Detailed information of TA system TA077450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3000587..3001218
• Replicon: chromosome
• Accession: NZ_CP021800
• Organism: Sinorhizobium meliloti strain USDA1021

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO29_RS14905
• Protein ID: WP_013844828.1
• Coordinates: 3000820..3001218 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92MD9
• Locus tag: CDO29_RS14900
• Protein ID: WP_010970129.1
• Coordinates: 3000587..3000820 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO29_RS14880	2996335..2996799	-	465	WP_010970133.1	Lrp/AsnC ligand binding domain-containing protein	-
CDO29_RS14885	2996822..2997985	-	1164	WP_010970132.1	amidohydrolase	-
CDO29_RS14890	2998237..2999319	+	1083	WP_010970131.1	D-alanyl-D-alanine carboxypeptidase	-
CDO29_RS14895	2999333..3000478	+	1146	WP_010970130.1	GTP-binding protein	-
CDO29_RS14900	3000587..3000820	+	234	WP_010970129.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDO29_RS14905	3000820..3001218	+	399	WP_013844828.1	type II toxin-antitoxin system toxin VapC	Toxin
CDO29_RS14910	3001252..3001491	-	240	WP_010970127.1	sulfurtransferase TusA family protein	-
CDO29_RS14915	3001491..3002933	-	1443	WP_010970126.1	murein L,D-transpeptidase	-
CDO29_RS14920	3003178..3004131	-	954	WP_003527479.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
CDO29_RS14925	3004195..3005130	-	936	WP_010970125.1	site-specific tyrosine recombinase XerD	-
CDO29_RS14930	3005127..3005276	-	150	WP_010970124.1	hypothetical protein	-
CDO29_RS14935	3005562..3005837	+	276	WP_010970123.1	type II toxin-antitoxin system ParD family antitoxin	-
CDO29_RS14940	3005834..3006136	+	303	WP_010970122.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14829.13 Da
• Isoelectric Point: 6.9581

>T77450 WP_013844828.1 NZ_CP021800:3000820-3001218 [Sinorhizobium meliloti]
MLTYMLDTNICIYVMKTYPPAVREKFNGLAEQLCISSITLGELHYGAEKSARRVENLTAIEHFVARLEVLPFADKAAAHY
GQVRAELERTGTPCGPHDMQIGAHARSEGLIVVTNNIREFVRMPGVRVENWL

Nucleotide

• Length: 399 bp

>T77450 NZ_CP021800:3000820-3001218 [Sinorhizobium meliloti]
ATGCTCACCTATATGCTCGATACCAATATCTGCATCTATGTGATGAAGACCTATCCGCCGGCCGTGCGCGAAAAGTTCAA
CGGACTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTGGGAGAATTACACTATGGCGCCGAAAAATCGGCCCGGCGCG
TTGAGAACCTTACGGCTATCGAACATTTCGTGGCACGGCTGGAGGTGCTGCCGTTCGCAGACAAGGCAGCGGCCCACTAC
GGGCAGGTTCGGGCTGAACTGGAGCGGACGGGAACGCCATGCGGCCCTCATGACATGCAGATCGGCGCGCATGCGCGCAG
CGAAGGGCTGATTGTCGTGACCAACAATATACGCGAGTTCGTCCGCATGCCGGGTGTAAGGGTCGAGAACTGGCTGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8673.87 Da
• Isoelectric Point: 8.9167

>AT77450 WP_010970129.1 NZ_CP021800:3000587-3000820 [Sinorhizobium meliloti]
MASSTVFISNRSQAVRLPKAVAFPEGVHQVDILKIGRSRVIVPQGKRWDDLFLSGPRVSEDFMSERDQPVAETRESF

Nucleotide

• Length: 234 bp

>AT77450 NZ_CP021800:3000587-3000820 [Sinorhizobium meliloti]
TTGGCCAGTTCGACCGTATTCATCAGTAACCGTAGCCAGGCGGTGCGGCTGCCAAAGGCCGTCGCCTTTCCCGAGGGCGT
GCATCAGGTCGATATCCTCAAGATCGGCCGCAGCCGCGTGATCGTCCCCCAGGGCAAAAGATGGGACGATCTGTTCCTCA
GCGGGCCGCGCGTCAGCGAGGATTTCATGAGCGAGCGTGATCAGCCGGTGGCGGAAACACGCGAATCCTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q92MD9