77447

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-RHH
Location 1126036..1126610 Replicon chromosome
Accession NZ_CP021800
Organism Sinorhizobium meliloti strain USDA1021

Toxin (Protein)


Gene name vapC Uniprot ID Q92QN4
Locus tag CDO29_RS05785 Protein ID WP_010969154.1
Coordinates 1126245..1126610 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q92QN3
Locus tag CDO29_RS05780 Protein ID WP_010969155.1
Coordinates 1126036..1126251 (+) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO29_RS05760 1121962..1122648 - 687 WP_010969157.1 ABC transporter ATP-binding protein -
CDO29_RS05765 1122666..1123976 - 1311 WP_014529722.1 lipoprotein-releasing ABC transporter permease subunit -
CDO29_RS05770 1123997..1125325 - 1329 WP_003531873.1 proline--tRNA ligase -
CDO29_RS05775 1125741..1125968 + 228 WP_017264047.1 hypothetical protein -
CDO29_RS05780 1126036..1126251 + 216 WP_010969155.1 ribbon-helix-helix protein, CopG family Antitoxin
CDO29_RS05785 1126245..1126610 + 366 WP_010969154.1 type II toxin-antitoxin system VapC family toxin Toxin
CDO29_RS05790 1126697..1126966 - 270 WP_014526683.1 DUF1467 family protein -
CDO29_RS05795 1127106..1127510 - 405 WP_010969152.1 methylmalonyl-CoA epimerase -
CDO29_RS05800 1127569..1129236 - 1668 WP_010969151.1 ribonuclease J -
CDO29_RS05805 1129264..1130031 - 768 WP_014529723.1 biotin--[acetyl-CoA-carboxylase] ligase -
CDO29_RS05810 1130028..1131470 - 1443 WP_010969150.1 NADH-quinone oxidoreductase subunit NuoN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13592.57 Da        Isoelectric Point: 5.1787

>T77447 WP_010969154.1 NZ_CP021800:1126245-1126610 [Sinorhizobium meliloti]
MVGALFDTNILIDHLNAVPQAHKELDRFENRAISIITWMEVMVGADAELVEPTRRFLDGFETIALNDEIANRAVTLRRAH
RIKLPDAVIWATAQTAGRLLVTRNTKDFPADDPGIREPYAV

Download         Length: 366 bp

>T77447 NZ_CP021800:1126245-1126610 [Sinorhizobium meliloti]
ATGGTAGGCGCACTGTTCGATACCAATATCCTTATCGATCATCTGAATGCGGTGCCTCAGGCGCACAAGGAACTTGACCG
TTTCGAAAACCGGGCCATCAGCATCATCACCTGGATGGAGGTGATGGTTGGAGCGGACGCCGAACTGGTTGAGCCGACCC
GGCGCTTCCTCGACGGTTTCGAAACGATCGCCTTGAACGATGAGATCGCCAACCGTGCGGTGACATTGCGCAGGGCGCAC
CGCATCAAGCTACCCGACGCGGTGATCTGGGCAACGGCGCAGACTGCCGGTCGTCTGCTCGTGACGCGCAATACCAAGGA
CTTTCCAGCCGACGACCCGGGGATACGCGAACCCTACGCCGTGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8381.51 Da        Isoelectric Point: 8.6052

>AT77447 WP_010969155.1 NZ_CP021800:1126036-1126251 [Sinorhizobium meliloti]
MRALIDMNDTQVEALDTLAKRVRRSRAALIREAIDDYLNRHHREQIEDGFGLWGKRKVDGLAYQEKVRGEW

Download         Length: 216 bp

>AT77447 NZ_CP021800:1126036-1126251 [Sinorhizobium meliloti]
ATGCGAGCCTTGATCGATATGAACGATACCCAGGTCGAAGCCCTAGATACCCTCGCCAAGCGGGTGCGCCGGTCCCGTGC
GGCTCTGATCCGCGAAGCGATCGACGACTATCTCAACCGTCATCATCGCGAGCAGATCGAAGATGGCTTCGGTTTGTGGG
GCAAGCGCAAAGTTGACGGTCTGGCTTATCAGGAGAAGGTGCGCGGCGAATGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92QN4

Antitoxin

Source ID Structure
AlphaFold DB Q92QN3

References