Detailed information of TA system TA077446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 323464..324118
• Replicon: chromosome
• Accession: NZ_CP021800
• Organism: Sinorhizobium meliloti strain USDA1021

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CDO29_RS01670
• Protein ID: WP_088203393.1
• Coordinates: 323930..324118 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: CDO29_RS01665
• Protein ID: WP_010969659.1
• Coordinates: 323464..323925 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO29_RS01625	318496..318861	+	366	WP_088203162.1	hypothetical protein	-
CDO29_RS01635	319506..319709	+	204	WP_088203391.1	hypothetical protein	-
CDO29_RS01640	320523..320696	+	174	WP_088203163.1	DUF3606 domain-containing protein	-
CDO29_RS01645	320840..321208	-	369	WP_088203164.1	hypothetical protein	-
CDO29_RS36885	321201..321371	-	171	WP_088203392.1	hypothetical protein	-
CDO29_RS01655	321465..322439	+	975	WP_164832948.1	non-homologous end-joining DNA ligase	-
CDO29_RS01660	322448..322867	-	420	WP_088203166.1	hypothetical protein	-
CDO29_RS01665	323464..323925	-	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CDO29_RS01670	323930..324118	-	189	WP_088203393.1	type II toxin-antitoxin system HicA family toxin	Toxin
CDO29_RS01675	324184..325403	+	1220	WP_088203167.1	IS3 family transposase	-
CDO29_RS01680	325630..326400	+	771	WP_010969658.1	hypothetical protein	-
CDO29_RS01685	326581..327552	+	972	WP_017265202.1	glycosyltransferase family 2 protein	-
CDO29_RS01690	327577..328887	-	1311	WP_010969656.1	HlyD family type I secretion periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	269953..325403	55450

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6975.13 Da
• Isoelectric Point: 11.2377

>T77446 WP_088203393.1 NZ_CP021800:c324118-323930 [Sinorhizobium meliloti]
IQSGDIIAALQKHGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T77446 NZ_CP021800:c324118-323930 [Sinorhizobium meliloti]
ATTCAGAGCGGCGACATTATTGCAGCATTGCAGAAGCACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT77446 WP_010969659.1 NZ_CP021800:c323925-323464 [Sinorhizobium meliloti]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT77446 NZ_CP021800:c323925-323464 [Sinorhizobium meliloti]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA

Structures

Antitoxin

• AlphaFold DB: Q92NX0