Detailed information of TA system TA077434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 975770..976347
• Replicon: chromosome
• Accession: NZ_CP021797
• Organism: Sinorhizobium meliloti strain USDA1106

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q92S20
• Locus tag: CDO30_RS04900
• Protein ID: WP_010968707.1
• Coordinates: 976084..976347 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: CDO30_RS04895
• Protein ID: WP_010968708.1
• Coordinates: 975770..976087 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO30_RS04865	971246..971635	+	390	WP_010968712.1	DUF930 domain-containing protein	-
CDO30_RS04870	971915..972226	-	312	WP_004435964.1	hypothetical protein	-
CDO30_RS04875	972429..973511	-	1083	WP_010968711.1	nucleotidyltransferase family protein	-
CDO30_RS04880	973498..974598	-	1101	WP_010968710.1	hypothetical protein	-
CDO30_RS04885	974918..975106	+	189	WP_013844020.1	hypothetical protein	-
CDO30_RS04890	975103..975567	+	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
CDO30_RS04895	975770..976087	-	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
CDO30_RS04900	976084..976347	-	264	WP_010968707.1	BrnT family toxin	Toxin
CDO30_RS04910	976931..977143	-	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
CDO30_RS04915	977148..977768	-	621	WP_004435951.1	Maf-like protein	-
CDO30_RS04920	977866..978084	-	219	WP_004435948.1	translation initiation factor IF-1	-
CDO30_RS04925	978242..978721	-	480	WP_010968705.1	low molecular weight phosphatase family protein	-
CDO30_RS04930	978735..979211	-	477	WP_010968704.1	UPF0262 family protein	-
CDO30_RS04935	979208..980512	-	1305	WP_010968703.1	histidinol dehydrogenase	-
CDO30_RS04940	980577..981017	-	441	WP_010968702.1	DUF2948 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T77434 WP_010968707.1 NZ_CP021797:c976347-976084 [Sinorhizobium meliloti]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASALIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T77434 NZ_CP021797:c976347-976084 [Sinorhizobium meliloti]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCCTCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT77434 WP_010968708.1 NZ_CP021797:c976087-975770 [Sinorhizobium meliloti]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT77434 NZ_CP021797:c976087-975770 [Sinorhizobium meliloti]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCGAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Structures

Toxin

• AlphaFold DB: Q92S20

Antitoxin

• AlphaFold DB: Q92KN5