Detailed information of TA system TA077430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 1670738..1671670
• Replicon: plasmid psymB
• Accession: NZ_CP021795
• Organism: Sinorhizobium meliloti strain USDA1157

Toxin (Protein)

• Gene name: -
• Uniprot ID: F7XGP1
• Locus tag: CDO31_RS35060
• Protein ID: WP_014530511.1
• Coordinates: 1671116..1671670 (+)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: CDO31_RS35055
• Protein ID: WP_017272317.1
• Coordinates: 1670738..1671109 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO31_RS35035	1666625..1667008	+	384	WP_010975573.1	RidA family protein	-
CDO31_RS35040	1667025..1669013	+	1989	WP_017272318.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
CDO31_RS35045	1669010..1669879	+	870	WP_014530514.1	hydroxymethylglutaryl-CoA lyase	-
CDO31_RS35050	1669876..1670661	+	786	WP_014530513.1	crotonase/enoyl-CoA hydratase family protein	-
CDO31_RS35055	1670738..1671109	+	372	WP_017272317.1	DUF2384 domain-containing protein	Antitoxin
CDO31_RS35060	1671116..1671670	+	555	WP_014530511.1	RES family NAD+ phosphorylase	Toxin
CDO31_RS35065	1671806..1673176	-	1371	WP_017272316.1	FAD-binding oxidoreductase	-
CDO31_RS35070	1673288..1674394	-	1107	WP_015008366.1	sulfate/molybdate ABC transporter ATP-binding protein	-
CDO31_RS35075	1674410..1675285	-	876	WP_013850796.1	sulfate ABC transporter permease subunit CysW	-
CDO31_RS35080	1675275..1676141	-	867	WP_015008365.1	sulfate ABC transporter permease subunit CysT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1700327	1700327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (23-177)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20449.31 Da
• Isoelectric Point: 6.4901

>T77430 WP_014530511.1 NZ_CP021795:1671116-1671670 [Sinorhizobium meliloti]
MRKHIPPASRPDSEPTSPVEPITLWRAFVPRWAHMPLSGAGAARFGGRWNPIGMPAIYAARELSTAWAEYNQGFVQHPAL
IVQLELRGARLADLTDASVLLELGVDEAIHRCEWRDALDKGAVPETHRLQSELLARDYHGVIYPSFMSPGGTCVALWRWN
GAEEPRLDVIDPDGRLPKSPASWV

Nucleotide

• Length: 555 bp

>T77430 NZ_CP021795:1671116-1671670 [Sinorhizobium meliloti]
ATGAGGAAACACATTCCGCCCGCATCGCGACCCGATTCGGAACCGACGTCGCCGGTCGAGCCGATAACGCTCTGGCGCGC
CTTCGTTCCGCGCTGGGCACATATGCCGCTATCCGGCGCGGGTGCCGCTCGCTTCGGTGGCCGCTGGAACCCGATCGGCA
TGCCTGCGATTTATGCTGCGCGCGAGCTTTCCACTGCCTGGGCTGAATATAATCAGGGTTTCGTCCAGCATCCGGCGCTC
ATCGTGCAGCTCGAGTTGCGCGGGGCGAGGCTCGCGGACCTCACGGATGCCAGCGTTCTTCTGGAGCTTGGTGTGGATGA
GGCGATCCATCGCTGCGAATGGCGTGATGCGCTCGACAAAGGCGCGGTGCCGGAGACCCACAGGCTGCAGTCGGAGCTTC
TGGCGCGCGACTATCACGGGGTGATCTATCCATCCTTCATGTCGCCCGGCGGCACCTGCGTCGCGCTCTGGCGCTGGAAC
GGTGCGGAAGAACCGCGTCTCGACGTCATCGATCCCGATGGCCGGCTGCCGAAATCGCCCGCCTCATGGGTTTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13103.22 Da
• Isoelectric Point: 9.6233

>AT77430 WP_017272317.1 NZ_CP021795:1670738-1671109 [Sinorhizobium meliloti]
MMAVDFQIAAARFGDDHSPFLSARLVADWLGVTLAELAKLIGVARNTLTAKSGARKVDSALSKVVRILAMASEMAGDEAR
AVIWFKHQPIPGWGGKTAFDLVGEGKADKVLAYLESVRAGVYA

Nucleotide

• Length: 372 bp

>AT77430 NZ_CP021795:1670738-1671109 [Sinorhizobium meliloti]
ATGATGGCCGTTGATTTCCAGATAGCTGCAGCCCGTTTCGGGGACGATCATTCCCCGTTTCTCTCGGCCCGCCTTGTCGC
CGACTGGTTGGGCGTCACGCTCGCCGAACTCGCCAAGCTGATCGGTGTCGCCCGCAATACGCTGACGGCGAAATCCGGCG
CCCGGAAGGTCGACAGCGCCTTGAGCAAGGTCGTCCGCATCCTCGCCATGGCTTCCGAAATGGCGGGCGACGAAGCCCGC
GCCGTCATCTGGTTCAAGCACCAGCCTATCCCCGGCTGGGGCGGCAAGACGGCGTTCGATCTGGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAATCGGTACGTGCCGGCGTCTACGCTTAA

Structures

Toxin

• AlphaFold DB: A0A222K2B4