Detailed information of TA system TA077429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 988551..989085
• Replicon: plasmid psymB
• Accession: NZ_CP021795
• Organism: Sinorhizobium meliloti strain USDA1157

Toxin (Protein)

• Gene name: parE
• Uniprot ID: H0FXJ9
• Locus tag: CDO31_RS37945
• Protein ID: WP_003527848.1
• Coordinates: 988789..989085 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: CDO31_RS31670
• Protein ID: WP_003527850.1
• Coordinates: 988551..988799 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO31_RS31635	984021..985088	+	1068	WP_010974979.1	ABC transporter substrate-binding protein	-
CDO31_RS31640	985085..986128	+	1044	WP_010974980.1	iron ABC transporter permease	-
CDO31_RS31645	986125..986925	+	801	WP_014527655.1	ABC transporter ATP-binding protein	-
CDO31_RS31650	986929..987723	+	795	WP_088195070.1	methyltransferase domain-containing protein	-
CDO31_RS31655	987807..987932	-	126	Protein_889	IS481 family transposase	-
CDO31_RS31670	988551..988799	+	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CDO31_RS37945	988789..989085	+	297	WP_003527848.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO31_RS37950	989164..989424	-	261	Protein_892	helix-turn-helix domain-containing protein	-
CDO31_RS31680	989998..990396	+	399	WP_010974988.1	hypothetical protein	-
CDO31_RS31685	990563..990748	+	186	WP_014527652.1	hypothetical protein	-
CDO31_RS31690	990752..991198	-	447	WP_014527651.1	DNA-binding protein	-
CDO31_RS31695	991488..991697	+	210	WP_003527838.1	dodecin family protein	-
CDO31_RS37360	991855..991998	+	144	WP_153318136.1	hypothetical protein	-
CDO31_RS31700	992231..993610	+	1380	WP_027988292.1	sodium:alanine symporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1700327	1700327
	-	flank	IS/Tn	-	-	987807..987998	191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-93)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11463.13 Da
• Isoelectric Point: 10.6593

>T77429 WP_003527848.1 NZ_CP021795:988789-989085 [Sinorhizobium meliloti]
MATERPFRLAPAAKADLRKIWRYTARRWSLEQAETYQDQLYTAFEGLAAGTKKGRNVDVRPGYLKYPAGSHIVYFRDRGD
RIDIIRILHGRMDAQRHL

Nucleotide

• Length: 297 bp

>T77429 NZ_CP021795:988789-989085 [Sinorhizobium meliloti]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTGCCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGCAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGACCGGGTTATCTCAAATACCCGGCAGGATCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGGATGGATGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT77429 WP_003527850.1 NZ_CP021795:988551-988799 [Sinorhizobium meliloti]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT77429 NZ_CP021795:988551-988799 [Sinorhizobium meliloti]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CTTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Toxin

• AlphaFold DB: H0FXJ9

Antitoxin

• AlphaFold DB: H0FXK0