Detailed information of TA system TA077428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1015417..1015916
• Replicon: plasmid psymA
• Accession: NZ_CP021794
• Organism: Sinorhizobium meliloti strain USDA1157

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q92YA6
• Locus tag: CDO31_RS24740
• Protein ID: WP_010967939.1
• Coordinates: 1015743..1015916 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CDO31_RS37840
• Protein ID: WP_198389772.1
• Coordinates: 1015417..1015746 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO31_RS24700	1010531..1011343	+	813	WP_014529334.1	IS21-like element ISRm9 family helper ATPase IstB	-
CDO31_RS24705	1011630..1011986	-	357	Protein_978	LysR family transcriptional regulator	-
CDO31_RS24710	1012078..1012521	+	444	WP_010967942.1	PACE efflux transporter	-
CDO31_RS24715	1012638..1012976	+	339	WP_013845641.1	SDR family oxidoreductase	-
CDO31_RS37835	1012960..1013302	-	343	Protein_981	hypothetical protein	-
CDO31_RS24725	1013686..1015056	-	1371	WP_013845640.1	alpha/beta hydrolase	-
CDO31_RS24730	1015297..1015482	-	186	WP_013845639.1	hypothetical protein	-
CDO31_RS37840	1015417..1015746	+	330	WP_198389772.1	damage-inducible protein	Antitoxin
CDO31_RS24740	1015743..1015916	+	174	WP_010967939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO31_RS24745	1016014..1016319	-	306	WP_013845638.1	hypothetical protein	-
CDO31_RS24755	1016638..1016997	-	360	WP_013845635.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
CDO31_RS24760	1017592..1017843	+	252	WP_003534243.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
CDO31_RS24765	1017871..1018356	+	486	WP_014528881.1	YidB family protein	-
CDO31_RS24770	1018732..1020048	-	1317	WP_013845634.1	aspartate aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1433621	1433621

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-100)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (11-90)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6497.32 Da
• Isoelectric Point: 5.7733

>T77428 WP_010967939.1 NZ_CP021794:1015743-1015916 [Sinorhizobium meliloti]
MKLVWARYALDDRDAIFSYIERENPRAAVHVDEEVVSAGRPLDFPESRRPGRIAGTP

Nucleotide

• Length: 174 bp

>T77428 NZ_CP021794:1015743-1015916 [Sinorhizobium meliloti]
ATGAAGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACGCCATCTTCAGCTACATCGAACGAGAGAATCCGAGGGC
GGCGGTCCACGTTGACGAGGAGGTCGTCAGCGCTGGTCGTCCGCTGGACTTTCCCGAAAGCCGCCGCCCTGGCCGGATTG
CCGGAACTCCATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12052.71 Da
• Isoelectric Point: 11.4911

>AT77428 WP_198389772.1 NZ_CP021794:1015417-1015746 [Sinorhizobium meliloti]
VSERLLLIALLALGPFRASQRHTGRPALLRRGPQSAAGAVEEHFIQLRAPLWLITLGGFSHREAHDAWFRAKVLRALENT
RPDADDADADAHFQERRAAALRKAAAGDR

Nucleotide

• Length: 330 bp

>AT77428 NZ_CP021794:1015417-1015746 [Sinorhizobium meliloti]
GTGAGCGAGCGCCTTCTGCTCATCGCCCTCCTGGCCCTCGGTCCGTTCCGAGCCAGCCAGCGGCATACCGGTCGTCCTGC
TCTGCTACGGCGTGGCCCCCAATCGGCCGCCGGCGCGGTCGAGGAGCACTTCATCCAACTCCGCGCTCCGCTGTGGCTAA
TCACGCTCGGCGGTTTCAGCCACAGAGAGGCTCACGACGCTTGGTTCCGCGCCAAAGTTTTGCGGGCACTCGAGAACACT
CGACCCGATGCGGACGACGCCGACGCCGATGCGCACTTTCAAGAGCGCCGCGCCGCAGCTTTGCGCAAGGCTGCGGCGGG
TGATCGATGA

Structures

Toxin

• AlphaFold DB: Q92YA6