77427

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 869822..870429 Replicon plasmid psymA
Accession NZ_CP021794
Organism Sinorhizobium meliloti strain USDA1157

Toxin (Protein)


Gene name hicA Uniprot ID A0A0E0UJ28
Locus tag CDO31_RS23915 Protein ID WP_014528001.1
Coordinates 870175..870429 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID H0G1X1
Locus tag CDO31_RS23910 Protein ID WP_003530441.1
Coordinates 869822..870178 (-) Length 119 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO31_RS23885 865327..866277 + 951 WP_010968047.1 ABC transporter permease -
CDO31_RS23890 866307..866963 + 657 WP_014528005.1 SGNH/GDSL hydrolase family protein -
CDO31_RS23895 867331..867921 - 591 WP_014528004.1 PIN domain-containing protein -
CDO31_RS23900 867924..868382 - 459 WP_014528003.1 helix-turn-helix domain-containing protein -
CDO31_RS23905 868673..869386 - 714 WP_088194805.1 3-keto-5-aminohexanoate cleavage protein -
CDO31_RS23910 869822..870178 - 357 WP_003530441.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CDO31_RS23915 870175..870429 - 255 WP_014528001.1 type II toxin-antitoxin system HicA family toxin Toxin
CDO31_RS23920 870771..871658 - 888 WP_014528000.1 LysR family transcriptional regulator -
CDO31_RS23925 871747..872787 + 1041 WP_014527999.1 NAD(P)H-binding protein -
CDO31_RS37820 872911..873069 + 159 WP_017267400.1 hypothetical protein -
CDO31_RS37255 873104..873310 + 207 WP_153495115.1 hypothetical protein -
CDO31_RS23930 873362..874234 - 873 WP_014527996.1 LysR family transcriptional regulator -
CDO31_RS23935 874325..875029 + 705 WP_014527995.1 haloacid dehalogenase type II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB / htpB 1..1433621 1433621


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9246.58 Da        Isoelectric Point: 9.8124

>T77427 WP_014528001.1 NZ_CP021794:c870429-870175 [Sinorhizobium meliloti]
MNSKQRKTLAAVFKDPASGTIEWAAIENLLVAAGADVIEGNGSRVKVEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Download         Length: 255 bp

>T77427 NZ_CP021794:c870429-870175 [Sinorhizobium meliloti]
ATGAACAGCAAGCAGAGAAAGACCCTCGCGGCCGTATTCAAGGACCCGGCCTCGGGAACGATTGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGAGCGGACGTCATTGAGGGGAACGGTTCCCGCGTTAAGGTTGAAAAAGACGGCATCATTG
CGAGCTTTCATCGACCTCATCCGGACAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin


Download         Length: 119 a.a.        Molecular weight: 13236.85 Da        Isoelectric Point: 4.9920

>AT77427 WP_003530441.1 NZ_CP021794:c870178-869822 [Sinorhizobium meliloti]
MNTMTYNGYHARIEFDAEDEVFFGKIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAADHFAEARLSA

Download         Length: 357 bp

>AT77427 NZ_CP021794:c870178-869822 [Sinorhizobium meliloti]
ATGAACACCATGACCTACAACGGGTATCACGCTCGCATCGAATTCGACGCTGAGGACGAAGTGTTTTTCGGCAAGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCGGTCGATGACT
ACCTTGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTCAACCAGTGGGCAGAAGAGGTGCTGGAAGAAGC
CGCCGACCATTTTGCCGAGGCGCGCCTGTCGGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0UJ28

Antitoxin

Source ID Structure
AlphaFold DB H0G1X1

References