77426

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 460611..461191 Replicon plasmid psymA
Accession NZ_CP021794
Organism Sinorhizobium meliloti strain USDA1157

Toxin (Protein)


Gene name vapC Uniprot ID Q92XP1
Locus tag CDO31_RS21640 Protein ID WP_010968151.1
Coordinates 460808..461191 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID F7XCL0
Locus tag CDO31_RS21635 Protein ID WP_013851145.1
Coordinates 460611..460811 (+) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO31_RS21610 455732..456838 + 1107 WP_088194711.1 FAD-binding oxidoreductase -
CDO31_RS21615 456828..457142 + 315 WP_014528248.1 (2Fe-2S)-binding protein -
CDO31_RS21620 457139..458515 + 1377 WP_014989788.1 NAD(P)/FAD-dependent oxidoreductase -
CDO31_RS21625 458604..459377 - 774 WP_014528246.1 UTRA domain-containing protein -
CDO31_RS21630 459556..460095 - 540 WP_088194712.1 TIGR02444 family protein -
CDO31_RS21635 460611..460811 + 201 WP_013851145.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CDO31_RS21640 460808..461191 + 384 WP_010968151.1 type II toxin-antitoxin system VapC family toxin Toxin
CDO31_RS21645 461639..462034 + 396 WP_010967157.1 response regulator -
CDO31_RS21650 462323..462634 - 312 WP_014990321.1 hypothetical protein -
CDO31_RS21655 462966..463801 + 836 Protein_442 TIGR02587 family membrane protein -
CDO31_RS21660 463798..464244 + 447 WP_017269446.1 TIGR02588 family protein -
CDO31_RS37740 464347..464904 + 558 Protein_444 DUF433 domain-containing protein -
CDO31_RS21670 464901..465152 + 252 Protein_445 DUF5615 family PIN-like protein -
CDO31_RS21680 465502..465906 - 405 WP_088194713.1 MerR family DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB / htpB 1..1433621 1433621


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14292.54 Da        Isoelectric Point: 6.9172

>T77426 WP_010968151.1 NZ_CP021794:460808-461191 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Download         Length: 384 bp

>T77426 NZ_CP021794:460808-461191 [Sinorhizobium meliloti]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7096.22 Da        Isoelectric Point: 11.1107

>AT77426 WP_013851145.1 NZ_CP021794:460611-460811 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT77426 NZ_CP021794:460611-460811 [Sinorhizobium meliloti]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92XP1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6MID2

References