Detailed information of TA system TA077425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 356917..357515
• Replicon: plasmid psymA
• Accession: NZ_CP021794
• Organism: Sinorhizobium meliloti strain USDA1157

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0E0UKM8
• Locus tag: CDO31_RS21050
• Protein ID: WP_014528329.1
• Coordinates: 357222..357515 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q930F1
• Locus tag: CDO31_RS21045
• Protein ID: WP_010967242.1
• Coordinates: 356917..357225 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO31_RS21010	352788..353042	+	255	Protein_331	nuclear transport factor 2 family protein	-
CDO31_RS21015	353074..353502	-	429	WP_017267827.1	VOC family protein	-
CDO31_RS21020	353610..353999	-	390	WP_014528333.1	hypothetical protein	-
CDO31_RS21025	354058..354678	-	621	WP_014528332.1	glutathione S-transferase C-terminal domain-containing protein	-
CDO31_RS21030	354790..355731	-	942	WP_014528331.1	helix-turn-helix domain-containing protein	-
CDO31_RS21035	355913..356371	+	459	WP_014528330.1	SRPBCC domain-containing protein	-
CDO31_RS21045	356917..357225	+	309	WP_010967242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CDO31_RS21050	357222..357515	+	294	WP_014528329.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
CDO31_RS21055	357777..358976	-	1200	WP_014528328.1	NAD-dependent formate dehydrogenase	-
CDO31_RS21060	359394..359807	-	414	WP_014528327.1	nuclear transport factor 2 family protein	-
CDO31_RS21065	359847..360329	+	483	WP_088194684.1	Lrp/AsnC family transcriptional regulator	-
CDO31_RS21070	360381..360812	+	432	WP_010967274.1	methyltransferase domain-containing protein	-
CDO31_RS21080	361499..361744	+	246	WP_014528323.1	membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1433621	1433621
	-	flank	IS/Tn	-	-	361999..363201	1202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11292.96 Da
• Isoelectric Point: 6.4734

>T77425 WP_014528329.1 NZ_CP021794:357222-357515 [Sinorhizobium meliloti]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAATRELVIPRTPYIAAYMVMEDRIRI
LRVLHGAQKWPSELDDG

Nucleotide

• Length: 294 bp

>T77425 NZ_CP021794:357222-357515 [Sinorhizobium meliloti]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGCAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11210.58 Da
• Isoelectric Point: 5.6604

>AT77425 WP_010967242.1 NZ_CP021794:356917-357225 [Sinorhizobium meliloti]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAALRKAAAGDR

Nucleotide

• Length: 309 bp

>AT77425 NZ_CP021794:356917-357225 [Sinorhizobium meliloti]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Structures

Toxin

• AlphaFold DB: A0A0E0UKM8

Antitoxin

• AlphaFold DB: Q930F1