77421

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacT-ataR/DUF1778(antitoxin)
Location 2638051..2638817 Replicon chromosome
Accession NZ_CP021793
Organism Sinorhizobium meliloti strain USDA1157

Toxin (Protein)


Gene name tacT Uniprot ID H0G6D3
Locus tag CDO31_RS13630 Protein ID WP_003533488.1
Coordinates 2638335..2638817 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID H0G6D4
Locus tag CDO31_RS13625 Protein ID WP_003533490.1
Coordinates 2638051..2638335 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO31_RS13610 2633521..2634909 + 1389 WP_003533497.1 lipopolysaccharide biosynthesis protein -
CDO31_RS13615 2634913..2636184 + 1272 WP_010970087.1 GNAT family N-acetyltransferase -
CDO31_RS13620 2636200..2637846 - 1647 WP_010970088.1 AMP-binding protein -
CDO31_RS13625 2638051..2638335 + 285 WP_003533490.1 DUF1778 domain-containing protein Antitoxin
CDO31_RS13630 2638335..2638817 + 483 WP_003533488.1 GNAT family N-acetyltransferase Toxin
CDO31_RS13640 2639282..2639791 + 510 WP_013844819.1 disulfide bond formation protein B -
CDO31_RS13645 2639823..2640380 - 558 WP_003533486.1 HNH endonuclease -
CDO31_RS13650 2640486..2641133 - 648 WP_010970091.1 DNA-3-methyladenine glycosylase -
CDO31_RS13655 2641286..2642182 + 897 WP_010970092.1 tRNA glutamyl-Q(34) synthetase GluQRS -
CDO31_RS13660 2642201..2643076 - 876 WP_010970093.1 YihY/virulence factor BrkB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-142)

Antitoxin

Vibrio phage ICP1, Orf50

(1-92)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17463.88 Da        Isoelectric Point: 7.3827

>T77421 WP_003533488.1 NZ_CP021793:2638335-2638817 [Sinorhizobium meliloti]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Download         Length: 483 bp

>T77421 NZ_CP021793:2638335-2638817 [Sinorhizobium meliloti]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGACACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10771.12 Da        Isoelectric Point: 7.3175

>AT77421 WP_003533490.1 NZ_CP021793:2638051-2638335 [Sinorhizobium meliloti]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Download         Length: 285 bp

>AT77421 NZ_CP021793:2638051-2638335 [Sinorhizobium meliloti]
ATGGCCACGAAATCGTCGACTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H0G6D3

Antitoxin

Source ID Structure
AlphaFold DB H0G6D4

References