Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 2406750..2407423 Replicon chromosome
Accession NZ_CP021793
Organism Sinorhizobium meliloti strain USDA1157

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag CDO31_RS12495 Protein ID WP_010969926.1
Coordinates 2407019..2407423 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID H0GA10
Locus tag CDO31_RS12490 Protein ID WP_003536345.1
Coordinates 2406750..2407022 (+) Length 91 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO31_RS12470 2402741..2404381 - 1641 WP_003536336.1 ABC transporter substrate-binding protein -
CDO31_RS12475 2404429..2405484 - 1056 WP_088194531.1 dipeptidase -
CDO31_RS12480 2405664..2406005 - 342 WP_003536339.1 EamA family transporter -
CDO31_RS12485 2406053..2406613 - 561 WP_010969925.1 TetR/AcrR family transcriptional regulator -
CDO31_RS12490 2406750..2407022 + 273 WP_003536345.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CDO31_RS12495 2407019..2407423 + 405 WP_010969926.1 type II toxin-antitoxin system VapC family toxin Toxin
CDO31_RS12500 2407551..2407943 + 393 WP_003536349.1 globin -
CDO31_RS12505 2407949..2408323 + 375 WP_010969927.1 DUF423 domain-containing protein -
CDO31_RS12510 2408446..2408706 + 261 WP_010969928.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CDO31_RS12515 2408821..2409135 - 315 WP_003536354.1 antibiotic biosynthesis monooxygenase -
CDO31_RS12520 2409148..2409414 - 267 WP_010969929.1 hypothetical protein -
CDO31_RS12525 2409467..2409880 - 414 WP_003536357.1 DUF2325 domain-containing protein -
CDO31_RS12530 2410088..2411014 - 927 WP_010969930.1 energy transducer TonB -
CDO31_RS12535 2411055..2411489 - 435 WP_010969931.1 hypothetical protein -
CDO31_RS12540 2411719..2411859 + 141 WP_010969932.1 hemin uptake protein HemP -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-124)

Antitoxin

(16-54)


Sequences


Toxin        


Download         Length: 135 a.a.        Molecular weight: 14747.98 Da        Isoelectric Point: 5.1974

>T77420 WP_010969926.1 NZ_CP021793:2407019-2407423 [Sinorhizobium meliloti]
MNGYLLDTNIISDVIHNPFGPAAQRIERIGPKEIYTSIVVASELRYGCAKKGSAKLLAKVESLLEIVPVLPLDIPADTRY
GSIRAELESLGQTIGSNDLLIAAHAYALDLTLVTDNIREFSRVRGLSLENWLER

Download         Length: 405 bp

>T77420 NZ_CP021793:2407019-2407423 [Sinorhizobium meliloti]
TTGAACGGCTATCTTCTCGATACGAACATCATCAGCGACGTCATTCACAATCCGTTCGGACCGGCTGCGCAACGCATCGA
ACGGATCGGCCCGAAGGAGATTTACACCAGCATCGTCGTCGCGTCCGAGTTGCGGTATGGTTGCGCAAAAAAGGGATCGG
CGAAGTTGCTCGCCAAGGTCGAAAGCTTGCTGGAAATCGTCCCGGTGCTGCCATTGGATATTCCGGCCGACACCCGATAT
GGCAGCATTCGTGCGGAATTGGAGTCTCTGGGGCAGACCATCGGCTCCAACGATCTGTTGATTGCCGCGCATGCATATGC
GCTGGACCTGACGCTGGTGACGGACAATATCCGTGAGTTCAGCCGCGTTCGTGGCCTTAGTCTGGAAAACTGGCTTGAGC
GATAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10334.02 Da        Isoelectric Point: 6.3950

>AT77420 WP_003536345.1 NZ_CP021793:2406750-2407022 [Sinorhizobium meliloti]
MPVPLPSSRPKEVKLFRNNRSQAVRIPAEFELPGDRVLIRREGTRLIIEPIARPADIVELLAEWKKEAPLGPEDRFPDVE
DIPARPEKIF

Download         Length: 273 bp

>AT77420 NZ_CP021793:2406750-2407022 [Sinorhizobium meliloti]
ATGCCCGTCCCGTTGCCGTCATCGCGACCTAAAGAAGTAAAGCTGTTCCGCAATAACCGCAGCCAGGCAGTCCGTATTCC
TGCGGAGTTCGAGTTGCCTGGAGACCGTGTGTTGATCCGTCGCGAAGGAACGAGACTCATTATCGAACCGATAGCCAGGC
CAGCCGATATCGTGGAGCTTCTCGCGGAGTGGAAGAAGGAAGCTCCCCTCGGACCTGAAGACCGGTTCCCGGATGTTGAG
GATATACCCGCCCGGCCGGAGAAAATCTTTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB H0GA10

References