Detailed information of TA system TA077419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 1943450..1944104
• Replicon: chromosome
• Accession: NZ_CP021793
• Organism: Sinorhizobium meliloti strain USDA1157

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: CDO31_RS10110
• Protein ID: WP_003530010.1
• Coordinates: 1943450..1943638 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: CDO31_RS10115
• Protein ID: WP_010969659.1
• Coordinates: 1943643..1944104 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO31_RS10095	1940016..1941326	+	1311	WP_010969656.1	HlyD family type I secretion periplasmic adaptor subunit	-
CDO31_RS10100	1941351..1942322	-	972	WP_017265202.1	glycosyltransferase family 2 protein	-
CDO31_RS10105	1942503..1943273	-	771	WP_010969658.1	hypothetical protein	-
CDO31_RS10110	1943450..1943638	+	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
CDO31_RS10115	1943643..1944104	+	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CDO31_RS10120	1944464..1945375	-	912	WP_088194449.1	PhnA-like protein	-
CDO31_RS10125	1945525..1946559	-	1035	WP_088194450.1	non-homologous end-joining DNA ligase	-
CDO31_RS10140	1947430..1947948	-	519	WP_088194451.1	hypothetical protein	-
CDO31_RS10145	1948065..1948487	-	423	WP_088194452.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T77419 WP_003530010.1 NZ_CP021793:1943450-1943638 [Sinorhizobium meliloti]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T77419 NZ_CP021793:1943450-1943638 [Sinorhizobium meliloti]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT77419 WP_010969659.1 NZ_CP021793:1943643-1944104 [Sinorhizobium meliloti]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT77419 NZ_CP021793:1943643-1944104 [Sinorhizobium meliloti]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA

Structures

Toxin

• AlphaFold DB: H0G1A3

Antitoxin

• AlphaFold DB: Q92NX0