Detailed information of TA system TA077415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 268464..269041
• Replicon: chromosome
• Accession: NZ_CP021793
• Organism: Sinorhizobium meliloti strain USDA1157

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q92S20
• Locus tag: CDO31_RS01325
• Protein ID: WP_010968707.1
• Coordinates: 268464..268727 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: CDO31_RS01330
• Protein ID: WP_010968708.1
• Coordinates: 268724..269041 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO31_RS01285	263793..264233	+	441	WP_010968702.1	DUF2948 family protein	-
CDO31_RS01290	264298..265602	+	1305	WP_010968703.1	histidinol dehydrogenase	-
CDO31_RS01295	265599..266075	+	477	WP_010968704.1	UPF0262 family protein	-
CDO31_RS01300	266089..266568	+	480	WP_010968705.1	low molecular weight phosphatase family protein	-
CDO31_RS01305	266726..266944	+	219	WP_004435948.1	translation initiation factor IF-1	-
CDO31_RS01310	267042..267662	+	621	WP_004435951.1	Maf-like protein	-
CDO31_RS01315	267667..267879	+	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
CDO31_RS01325	268464..268727	+	264	WP_010968707.1	BrnT family toxin	Toxin
CDO31_RS01330	268724..269041	+	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
CDO31_RS01335	269244..269708	-	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
CDO31_RS01340	269705..269893	-	189	WP_013844020.1	hypothetical protein	-
CDO31_RS01345	270213..271313	+	1101	WP_010968710.1	hypothetical protein	-
CDO31_RS01350	271300..272382	+	1083	WP_010968711.1	nucleotidyltransferase family protein	-
CDO31_RS01355	272585..272896	+	312	WP_004435964.1	hypothetical protein	-
CDO31_RS01360	273176..273565	-	390	WP_013844021.1	DUF930 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	253337..269893	16556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T77415 WP_010968707.1 NZ_CP021793:268464-268727 [Sinorhizobium meliloti]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASALIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T77415 NZ_CP021793:268464-268727 [Sinorhizobium meliloti]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCCTCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT77415 WP_010968708.1 NZ_CP021793:268724-269041 [Sinorhizobium meliloti]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT77415 NZ_CP021793:268724-269041 [Sinorhizobium meliloti]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCAAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Structures

Toxin

• AlphaFold DB: Q92S20

Antitoxin

• AlphaFold DB: Q92KN5