Detailed information of TA system TA077401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 99534..100139
• Replicon: chromosome
• Accession: NZ_CP021780
• Organism: Paenibacillus donghaensis strain KCTC 13049

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0W1B4L6
• Locus tag: B9T62_RS00460
• Protein ID: WP_042124727.1
• Coordinates: 99789..100139 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: B9T62_RS00455
• Protein ID: WP_154117276.1
• Coordinates: 99534..99785 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9T62_RS00440	95108..96262	+	1155	WP_087913480.1	winged helix-turn-helix domain-containing protein	-
B9T62_RS00445	96844..97929	+	1086	WP_087913481.1	outer membrane lipoprotein carrier protein LolA	-
B9T62_RS00450	98110..99300	+	1191	WP_087913482.1	alanine racemase	-
B9T62_RS00455	99534..99785	+	252	WP_154117276.1	ribbon-helix-helix protein, CopG family	Antitoxin
B9T62_RS00460	99789..100139	+	351	WP_042124727.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B9T62_RS00465	100492..101013	+	522	WP_087920065.1	TetR/AcrR family transcriptional regulator	-
B9T62_RS00470	101247..102443	+	1197	WP_087913483.1	MFS transporter	-
B9T62_RS00475	102615..104777	+	2163	WP_211296482.1	helix-hairpin-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-35)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12764.68 Da
• Isoelectric Point: 5.1171

>T77401 WP_042124727.1 NZ_CP021780:99789-100139 [Paenibacillus donghaensis]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDETMKKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T77401 NZ_CP021780:99789-100139 [Paenibacillus donghaensis]
TTGATCGTTAAACGCGGCGACGTTTTTTTTGCCGACCTTTCACCGGTTGTAGGTTCAGAGCAAGGCGGGGTTAGGCCGGT
ACTGGTCATACAGAACGATATTGGCAATCGCTTCAGCCCGACAGTGATTGTGGCAGCCATTACTGCCCAGATTCAAAAGG
CTAAACTGCCGACGCATGTGGAAATTGATGCGGCTGCCCACGGTTTCGACAGAGATTCTGTTATCCTGCTGGAGCAGGTT
CGGACGATTGACAAGCAACGCTTGACCGATAAGATCACCCATCTTGATGATGAAACCATGAAGAAGGTGGATGATTCGCT
GCAAATCAGCCTGGGTCTGATTGATTTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9487.94 Da
• Isoelectric Point: 5.1184

>AT77401 WP_154117276.1 NZ_CP021780:99534-99785 [Paenibacillus donghaensis]
MISLPDHLLQEVDGIVQLENSNRSELIRQAMKLYLSERRKRSIRESMQRGYMEMAKINLTMACEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT77401 NZ_CP021780:99534-99785 [Paenibacillus donghaensis]
ATGATCAGCTTGCCCGATCATCTCTTGCAGGAAGTGGATGGAATCGTTCAACTGGAGAATTCCAACCGGAGTGAATTGAT
CAGGCAGGCCATGAAGCTGTACTTGAGTGAACGGAGGAAACGTTCTATCCGGGAGTCAATGCAGCGTGGCTACATGGAAA
TGGCTAAGATCAACTTAACCATGGCATGCGAGGCGTTCCTCGCCGAGGAAGATGCAGACAGCACTCTTGGCCGCTTAGTA
AGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A0W1B4L6