Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 25578..26221 Replicon plasmid unitig_3
Accession NZ_CP021778
Organism Enterobacter cloacae complex sp. strain AR_0053

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AM432_RS25315 Protein ID WP_001044770.1
Coordinates 25805..26221 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AM432_RS25310 Protein ID WP_001261282.1
Coordinates 25578..25808 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM432_RS25280 (AM432_25475) 21295..22050 - 756 WP_012539983.1 replication initiation protein RepE -
AM432_RS26075 22681..22794 + 114 WP_032072094.1 hypothetical protein -
AM432_RS25290 (AM432_25485) 22923..23180 + 258 WP_032408936.1 hypothetical protein -
AM432_RS25295 (AM432_25490) 23238..24023 - 786 WP_022644886.1 site-specific integrase -
AM432_RS25300 (AM432_25495) 24026..24664 - 639 WP_022644887.1 hypothetical protein -
AM432_RS25930 24713..25033 - 321 WP_032072060.1 hypothetical protein -
AM432_RS25305 (AM432_25500) 25161..25621 - 461 Protein_34 hypothetical protein -
AM432_RS25310 (AM432_25505) 25578..25808 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
AM432_RS25315 (AM432_25510) 25805..26221 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AM432_RS25320 (AM432_25515) 26295..26513 + 219 Protein_37 AAA family ATPase -
AM432_RS25325 (AM432_25520) 26578..27282 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
AM432_RS25330 (AM432_25525) 27335..28102 - 768 Protein_39 class 1 integron integrase IntI1 -
AM432_RS25335 (AM432_25535) 28258..28731 + 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
AM432_RS26380 28952..29104 + 153 Protein_41 plasmid mobilization relaxosome protein MobC -
AM432_RS26385 29178..29243 + 66 Protein_42 EAL domain-containing protein -
AM432_RS25350 (AM432_25555) 29361..30125 + 765 WP_001389365.1 IS6-like element IS6100 family transposase -
AM432_RS26080 30265..30330 - 66 Protein_44 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaKPC-3 - 1..71949 71949


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T77400 WP_001044770.1 NZ_CP021778:25805-26221 [Enterobacter cloacae complex sp.]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T77400 NZ_CP021778:25805-26221 [Enterobacter cloacae complex sp.]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT77400 WP_001261282.1 NZ_CP021778:25578-25808 [Enterobacter cloacae complex sp.]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT77400 NZ_CP021778:25578-25808 [Enterobacter cloacae complex sp.]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References