Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4606764..4607384 Replicon chromosome
Accession NZ_CP021776
Organism Enterobacter cloacae complex sp. strain AR_0053

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag AM432_RS22770 Protein ID WP_015571250.1
Coordinates 4606764..4606982 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag AM432_RS22775 Protein ID WP_006809850.1
Coordinates 4607010..4607384 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM432_RS22740 (AM432_22905) 4602776..4603036 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
AM432_RS22745 (AM432_22910) 4603039..4603179 + 141 WP_032608666.1 type B 50S ribosomal protein L36 -
AM432_RS22750 (AM432_22915) 4603176..4603886 - 711 WP_022650493.1 GNAT family protein -
AM432_RS22755 (AM432_22920) 4603988..4605448 + 1461 WP_032608667.1 PLP-dependent aminotransferase family protein -
AM432_RS22760 (AM432_22925) 4605420..4605887 - 468 WP_022650495.1 YlaC family protein -
AM432_RS22765 (AM432_22930) 4606004..4606555 - 552 WP_022650496.1 maltose O-acetyltransferase -
AM432_RS22770 (AM432_22935) 4606764..4606982 - 219 WP_015571250.1 HHA domain-containing protein Toxin
AM432_RS22775 (AM432_22940) 4607010..4607384 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
AM432_RS22780 (AM432_22945) 4607895..4611041 - 3147 WP_022650497.1 multidrug efflux RND transporter permease subunit AcrB -
AM432_RS22785 (AM432_22950) 4611064..4612257 - 1194 WP_022650498.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T77399 WP_015571250.1 NZ_CP021776:c4606982-4606764 [Enterobacter cloacae complex sp.]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T77399 NZ_CP021776:c4606982-4606764 [Enterobacter cloacae complex sp.]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT77399 WP_006809850.1 NZ_CP021776:c4607384-4607010 [Enterobacter cloacae complex sp.]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT77399 NZ_CP021776:c4607384-4607010 [Enterobacter cloacae complex sp.]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References