Detailed information of TA system TA077396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3168806..3169413
• Replicon: chromosome
• Accession: NZ_CP021776
• Organism: Enterobacter cloacae complex sp. strain AR_0053

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4Q4AAS0
• Locus tag: AM432_RS15800
• Protein ID: WP_057979982.1
• Coordinates: 3169228..3169413 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM432_RS15795
• Protein ID: WP_023315016.1
• Coordinates: 3168806..3169213 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM432_RS15780 (AM432_15850)	3165549..3166802	+	1254	WP_015572749.1	valine--pyruvate transaminase	-
AM432_RS15785 (AM432_15855)	3166810..3167265	-	456	WP_023323869.1	4Fe-4S dicluster domain-containing protein	-
AM432_RS15790 (AM432_15860)	3167726..3168802	+	1077	WP_023323870.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
AM432_RS15795 (AM432_15865)	3168806..3169213	-	408	WP_023315016.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AM432_RS15800 (AM432_15870)	3169228..3169413	-	186	WP_057979982.1	type II toxin-antitoxin system HicA family toxin	Toxin
AM432_RS15805 (AM432_15875)	3169663..3171201	-	1539	WP_006808612.1	aldehyde dehydrogenase AldB	-
AM432_RS15810 (AM432_15880)	3171368..3172252	+	885	WP_003860164.1	ROK family protein	-
AM432_RS15815 (AM432_15885)	3172256..3174094	-	1839	WP_023323871.1	selenocysteine-specific translation elongation factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6852.08 Da
• Isoelectric Point: 11.5191

>T77396 WP_057979982.1 NZ_CP021776:c3169413-3169228 [Enterobacter cloacae complex sp.]
VKSADIITVLVSHGWKCVRTKGSHHQFRHPVQKGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Nucleotide

• Length: 186 bp

>T77396 NZ_CP021776:c3169413-3169228 [Enterobacter cloacae complex sp.]
GTGAAAAGTGCGGATATCATTACTGTTCTGGTTAGCCATGGCTGGAAATGTGTAAGAACGAAGGGAAGCCATCATCAGTT
TCGCCATCCAGTGCAAAAAGGGTTGGTTACCGTCCCACATCCTAAAAAGGACATTAAACCAGGCACTCTCGCACAAATCT
GGCGACAGGCGGGAATTAAACACTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15205.20 Da
• Isoelectric Point: 4.5036

>AT77396 WP_023315016.1 NZ_CP021776:c3169213-3168806 [Enterobacter cloacae complex sp.]
MFYPAYIHSDSDGSASGFFPDVPGCFFAGDSLDDAFQDARDALTAHFEALFEMDEMLPLPGMVEAHLASHPEDFIGGQWL
LVDINMKQFEGRVERINITIPRRLLVKIDSFVSEHPQFTNRSAFLAEAARRVLPR

Nucleotide

• Length: 408 bp

>AT77396 NZ_CP021776:c3169213-3168806 [Enterobacter cloacae complex sp.]
ATGTTTTATCCGGCTTACATTCATTCTGATTCCGACGGTAGCGCCAGTGGTTTCTTTCCTGACGTTCCGGGGTGTTTTTT
TGCGGGGGATTCACTGGATGATGCTTTTCAGGATGCACGTGATGCATTAACTGCCCATTTTGAAGCGTTGTTTGAAATGG
ATGAAATGTTGCCACTTCCGGGTATGGTTGAAGCGCATCTGGCAAGTCATCCGGAGGATTTCATTGGCGGACAATGGTTG
CTGGTGGATATCAATATGAAACAGTTTGAAGGCAGAGTAGAGCGCATCAATATTACGATACCTCGCCGTTTGCTGGTGAA
GATTGATTCCTTTGTCAGCGAGCATCCACAATTTACTAACCGAAGTGCTTTTCTTGCAGAGGCAGCACGGCGTGTACTGC
CCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A4Q4AAS0