Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA(toxin)
Location 1612109..1613892 Replicon chromosome
Accession NZ_CP021776
Organism Enterobacter cloacae complex sp. strain AR_0053

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag AM432_RS07955 Protein ID WP_032609694.1
Coordinates 1612109..1613458 (+) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A837FFV9
Locus tag AM432_RS07960 Protein ID WP_003859317.1
Coordinates 1613455..1613892 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM432_RS07930 (AM432_07940) 1607932..1608867 - 936 WP_003859306.1 tRNA dihydrouridine(16) synthase DusC -
AM432_RS07935 (AM432_07945) 1609036..1609431 + 396 WP_003859308.1 CidA/LrgA family protein -
AM432_RS07940 (AM432_07950) 1609428..1610123 + 696 WP_003859310.1 CidB/LrgB family autolysis modulator -
AM432_RS07945 (AM432_07955) 1610252..1611136 + 885 WP_032609693.1 cytidine deaminase -
AM432_RS07950 (AM432_07960) 1611264..1611974 + 711 WP_003859314.1 outer membrane permeability protein SanA -
AM432_RS07955 (AM432_07965) 1612109..1613458 + 1350 WP_032609694.1 HipA domain-containing protein Toxin
AM432_RS07960 (AM432_07970) 1613455..1613892 + 438 WP_003859317.1 helix-turn-helix transcriptional regulator Antitoxin
AM432_RS07965 (AM432_07975) 1613931..1614941 - 1011 WP_003859318.1 galactose/methyl galactoside ABC transporter permease MglC -
AM432_RS07970 (AM432_07980) 1614957..1616477 - 1521 WP_003859320.1 galactose/methyl galactoside ABC transporter ATP-binding protein MglA -
AM432_RS07975 (AM432_07985) 1616557..1617555 - 999 WP_003859322.1 galactose/glucose ABC transporter substrate-binding protein MglB -
AM432_RS07980 (AM432_07990) 1617851..1618873 - 1023 WP_003859324.1 HTH-type transcriptional regulator GalS -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-406)

Antitoxin

Cro/C1-type helix-turn-helix domain

(75-128)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 51149.84 Da        Isoelectric Point: 4.6562

>T77393 WP_032609694.1 NZ_CP021776:1612109-1613458 [Enterobacter cloacae complex sp.]
METLTVQAYLNETWTDIALIKYPGSEKNDWNITQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHEINAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRALINKITSSHMVQQQGYHFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Download         Length: 1350 bp

>T77393 NZ_CP021776:1612109-1613458 [Enterobacter cloacae complex sp.]
ATGGAGACGTTAACCGTACAGGCATATCTGAACGAAACATGGACGGATATCGCTCTCATCAAATATCCTGGAAGTGAAAA
AAATGACTGGAATATCACGCAGTTAGACTATCTTACTGAGTATGCGATTAATTTTCTCGACTATGATGATTTTCACGCAG
TCTCAGTCAATCACCCTGTATCACTCTTTTTTGATGACCATGGCCAACCGGGCTGGCTGCGATTTATCGACGATATCATT
CCCGCCGGAGCAAGCCGCCGTTACTGGATTAATGCGCTCGACATCAGTGAGTTACCCGTTGGCCAGCAAAATTTCTTATT
GCTGAAATTTGGCACAATGTCCCCTGTGGGGAATTTGCGCATAAAGGAATCCGTCCCAGAATGGAATAAATTCGCCAGCA
CTAAAACGTTTACGGTTGCGGACGTCATCGATCGTGCGGCCGATTTTCTGGATTATGCCCAGGAAAGAGGCGCGGCGGCG
GGTGGTGCAACCGGTGCTGGCGGTGAAGCGCCTAAACTTTTGCTGCGCTGTAGTGAAACTGATGCCATCTGGATCGATAC
CTGGCAAAACGAGCCGGATAACCGGGACCGCTATTATTTGGTTAAATATCCACGCGGTAACAGGACAGAAGTGGATTGTA
ATATTCTGCGAGCTGAATTCCATTATTATCATGAGATTAACGCGATGGGTTTTTCCACGATCCCAGTGGAAACAATGCGC
CTTGAAGAAGGTAGCCATTACCCGTCTCTGTGGTTACCGCGTTTCGACATAGTGAGAAATGAGCAGGACCAACTGGTCAG
GCTGGGTATGGAATCGGTGTATTCGCTATTACAAAAGGCACCCGGCACCCTCCTTGATCATGAGACAACGTTGCGCGCGT
TAATTAATAAAATCACCTCCAGCCATATGGTCCAGCAGCAGGGTTATCATTTTGATATTCCGGGATTTGTAACAGAATGG
GTGCGTCGCGATCTGCTAAATATTATTTTTGGAAATAGTGACAACCATGGGCGAAATACCGCCTTTGTGAAGGCTGAAAA
TCAAATTATGCTCTCCCCTGTATATGACTTTGCGCCGATGAAAGCCGATCCTGAAGGGATACCAAGAACCCTGAAGTGGT
CATTATCTTGCGAATCAGGGGGGGATTATAATTTCAACACTATCGCACAAACGCTGGCGGAGTGGATCCCTCCCGCAACA
TTACTTGATGCTCTGCATGAAACAGCCGTTCAGCTTATTGATCTCCCGGAAAGGCTGGCGGCGCGAGGCGTTCCCGAACA
GATAATGGAAATGCCCGCGATGGGTTTTCGCTACATCCCGGATAAACTTTCCCGTTGGGGTTTATTATGA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16185.64 Da        Isoelectric Point: 10.5154

>AT77393 WP_003859317.1 NZ_CP021776:1613455-1613892 [Enterobacter cloacae complex sp.]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEEPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Download         Length: 438 bp

>AT77393 NZ_CP021776:1613455-1613892 [Enterobacter cloacae complex sp.]
ATGAAAACACCTAATGAAGCTGAATCAGAGCTTTTACAGACATTAGCTCAAGTACGCAGCGTAAATAAAAAGCGACATCA
TGACGAGGCGGAAGAGCCGGATAAGCCATCGGTAGTGAAGAGGCAACGCGTTACACAAGGCTTAACGCGGATTAGTACTC
TCGATCGCCAGGCTGTACTGCATGCAGCCATACGGGACATTTTGCTGGGGAAAATCACACAGGGAGAGGCGCTGAAAAGG
CTCAGGGTCGAGGTGTTAGGGCTGAAGCAGGATGAATATGCAAAGCTGGTCAGCGTATCCCGGAAAACATTGTCGGATGT
AGAAAATAACAGAGGCAATTATTCCGCTGACGTCATAAATAAAATCTTTAAACCCTTTGGGCTTCAAACCGGACTAGTGC
CCGTTTCAAAATCGCTTATCGCTTCACTTTTCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A837FFV9

References